垂穗披碱草MADS-box基因WM8克隆及分析  

Cloning and analysis of the MADS-box gene WM8 of Elymus nutans

作　　者：张妙青[1] 张吉宇[1] 刘志鹏[1] 王彦荣[1] 张磊[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2012年第4期141-150,共10页Acta Prataculturae Sinica

基　　金：国家"973"课题(编号:2007CB108904);兰州大学中央高校基本科研费专项基金(编号:lzujbky-2009-108;lzujbky-2009-4);"十一五"国家科技支撑计划子课题(编号:2008BADB3B03-4)资助

摘　　要：通过同源克隆从垂穗披碱草中获得了MADS-box基因家族WM8基因的全长cDNA序列,命名为EnWM8(Genbank登录号为JF683846)。序列分析表明,该cDNA全长1 196bp,开放阅读框共编码275个氨基酸,具有典型的MADS-box结构域以及半保守的K区,是MIKC型MADS-box基因,分析认为其与花发育和果实成熟等有关。EnWM8与小麦的WM8、AGL29和fruitful-like基因氨基酸的推导序列的相似性分别高达95.62%,96.72%和96.28%。采用DNAMAN软件进行系统进化树分析显示,EnWM8基因与小麦的TaWM8进化关系最近,与拟南芥的AtWM8、AtAGL29进化关系较远。垂穗披碱草WM8蛋白分子量为13 726.7Da,理论等电点为9.14,为亲水性的碱性蛋白,无跨膜结构域,定位于细胞核中,其空间结构主要由α-螺旋、无规则卷曲和延伸链构成。研究结果可为进一步从分子水平探明垂穗披碱草花发育、种子成熟等机制提供参考。The full length MADS-box gene EnWM8 was isolated from Elymus nutans by homology cloning,and was submitted to GenBank（accession number JF683846）.Analyses of the cDNA sequence showed that EnWM8 was 1 196 bp and encoded 275 amino acids,typical of the MIKC-type MADS-box genes with a typical MADS-box domain and K semi-conservative district.It is speculated that it has some connection with flower development,fruit ripening etc.The deduced amino acid sequence showed that there were 95.62%,96.72%,96.28% similarities between the gene and Triticum aestivum genes WM8,AGL29,and fruitful-like.The phylogenetic tree deduced by DNAMAN software indicated that EnWM8 had a close evolutionary relationship with the TaWM8 of T.aestivum,and was distantly related to the AtWM8 and AtAGL29 of Arabidopsis.The WM8 protein of E.nutans is a hydrophilic and basic protein with a molecular weight of 13 726.7 Da： the theoretical isoelectric point is 9.14.It has no membrane structure and is mainly located in the nucleus.There are many α-helix,random coil and extended strand structures.These results are a basis for further research on flower development,seed maturation and other mechanisms of E.nutans at the molecular level.

关 键 词：垂穗披碱草 MADS-BOX WM8基因 序列分析 蛋白结构 

分 类 号：S543.9[农业科学—作物学]