淀粉样前体蛋白转基因痴呆模型小鼠视网膜和视神经纤维层病理改变  

Pathological changes of retina and optic nerve fiber layer in amyloid precursor protein transgenic model mouse of Alzheimer’s disease

作　　者：卢艳[1] 董海莲[1] 张丽娜[2] 王蓉[3] 

机构地区：[1]首都医科大学宣武医院眼科,北京市100053 [2]郑州人民医院眼科,河南省郑州市450000 [3]首都医科大学宣武医院中心实验室,北京市100053

出　　处：《眼科新进展》2012年第9期810-813,共4页Recent Advances in Ophthalmology

基　　金：国家"十一五"863计划疫苗与抗体工程子课题(编号:2006AA02A245);北京市卫生系统高层次卫生技术人才培养计划(编号:2009-3-64)~~

摘　　要：目的观察淀粉样前体蛋白(amyloid precusor protein,APP)转基因痴呆(Alzheimer’s disease,AD)模型小鼠视网膜组织病理和视神经纤维记数的改变。方法本实验采用6只10月龄的C57BL/6JAPP转基因雄性小鼠模型作为模型组,6只同月龄的C57BL/6J正常雄性小鼠作为正常对照组。处死小鼠,完整取出眼球分离视网膜,进行视网膜常规HE染色,同时取球后视神经制作半薄切片,甲苯胺蓝染色,光镜观察视神经形态,行图像分析和轴突纤维计数。结果视神经横断面模型组小鼠与正常对照组相比:神经纤维组织疏松,排列较紊乱,染色深浅不均,间质增多,部分轴突水肿。与正常对照组(1.77±0.04)×106相比,模型组小鼠视神经纤维数量(2.28±0.06)×106明显减少,视神经纤维密度正常对照组和模型组分别为(0.3836±0.0697)μm-2和(0.2701±0.0517)μm-2,差异有统计学意义(P<0.01)。模型组神经细胞平均光密度值无明显变化,与正常对照组比较差异无统计学意义(P>0.05)。与正常对照组比较,模型组小鼠视网膜神经节细胞层细胞数量明显减少,差异有统计学意义(P<0.01),两组内颗粒层和外颗粒层细胞数量无明显变化,差异无统计学意义(均为P>0.05)。结论 APP转基因AD模型小鼠视网膜神经节细胞数减少,视神经纤维数明显减少而且受损,提示APP转基因AD模型小鼠视网膜和视神经存在退行性病变。Objective To observe the changes of retinal pathology and optic nerve fiber count in amyloid precursor protein （APP） transgenic model mouse of Alzheimer’s disease. Methods There were two groups in the study.Model group contained six APP transgenic male C57BL/6J mice with ten months old.Normal group were six normal male C57BL/6J mice with ten months old.First,APP transgenic mice with 10 months were sacrificed,and the whole eyeballs and retrobulbar optic nerve were dissected.The retinas were embedded with paraffin,mode slices,stained with HE to observe the retinal ganglion cells pathological changes with optical microscope.Retrobulbar optic nerves were stained with toluidine blue to count the axons of the optic nerve fiber with optical microscope. Results Morphological changes of the optic nerve section：Compared with the control group,in model group the nerve fibers were loose with disorder,the dying was uneven,the stroma was increased,and part of the axon was edema.The number of optic nerve fibers in control and model group were （2.28±0.06）×10^6 and （1.77±0.04）×10^6,respectively,the optic nerve fiber density were （0.383 6±0.069 7）μm^-2 and （0.270 1±0.051 7）μm^-2,respectively,there were significant differences between two groups（both P〈0.01）.The average optical density value of the nerve cells in model group was not evidently changed,there was no statistical difference compared with the control group（P〈0.05）.Compared with the control group,the number of retinal ganglion cells in model group were significantly reduced,there was significant difference（P〈0.01）.There was no obvious change in internal granular layer and external granular layer cells of two groups,there was no statistical difference（both P〈0.05）. Conclusion The number of retinal ganglion cells and optic nerve fibers of APP transgenic mice reduce,and the optic nerve fibers are damaged,which indicate that the retrogressive degeneration exist in retina and optic nerve in APP transgenic model mouse of A

关 键 词：淀粉样前体蛋白转基因痴呆模型小鼠 阿尔兹海默病 视网膜神经节细胞 

分 类 号：R775[医药卫生—眼科]