NLRP3炎性小体在EAE大鼠中的表达及意义  

Significance of the NLRP3 inflammasome expression in rats with experimental autoimmune encephalomyelitis

作　　者：银晓端[1] 曾丽[1] 周建波[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021

出　　处：《中风与神经疾病杂志》2012年第8期699-702,共4页Journal of Apoplexy and Nervous Diseases

基　　金：广西自然科学基金资助项目(桂科自0542088)

摘　　要：目的通过检测实验性自身免疫性脑脊髓炎(EAE)大鼠中NLRP3信号传导通路的改变,探讨NL-RP3炎性小体介导的释放IL-1β的炎性反应在EAE大鼠发病机制中的作用。方法将30只大鼠随机分为两组,正常对照组10只,EAE模型组20只,并以豚鼠脊髓匀浆(GPSCH)为抗原免疫建立EAE模型。免疫组化检测比较对照组和模型组大鼠CNS内NLRP3、caspase-1、IL-1β细胞因子的表达情况并进行研究分析。结果模型组脑和脊髓的NLRP3、caspase-1、IL-1β的表达均高于正常对照组(P<0.05)。结论 NLRP3介导炎性反应可能参与了EAE的发病过程,可能是MS的发病机制之一。Objective To detect changes of NLRP3 signal transduction pathway of experimental autoimmune encephalomyelitis（EAE） to analyze NLRP3-mediated IL-1β release inflammatory process in rats.Methods Rats were randomly divided into two groups,10 rats in the control group,20 rats in the model group.EAE model was induced in rats by immunization with guinea pig spinal cord homogenate（GPSCH）.The expression of NLRP3,caspase-1 and IL-1β in the CNS of two groups was detected by immunohistochemistry.Results The expression of NLRP3,caspase-1 and IL-1β in the model group was more than the control group in every part（P0.05）.Conclusion NLRP3-mediated inflammatory response probably involved in the process of the EAE,so it maybe one of the pathogenesis of multiple sclerosis.

关 键 词：多发性硬化 EAE 炎性小体 CASPASE-1 IL-1Β 

分 类 号：R742[医药卫生—神经病学与精神病学]