Endostatin抑制角膜新生血管及影响Flk-1表达的实验研究  

The experimental study about endostatin inhibiting corneal neovascularization and effects on the expression of ilk-1

作　　者：王平[1] 康凤英[1] 邓爱军[1] 李娜[1] 夏飞[1] 

机构地区：[1]潍坊医学院附属医院眼科中心,潍坊261031

出　　处：《中国实用眼科杂志》2012年第9期1115-1119,共5页Chinese Journal of Practical Ophthalmology

摘　　要：目的建立碱烧伤角膜新生血管（CNV）动物模型，研究内皮抑素Endostatin（ES）对大鼠CNV形成的抑制作用和它对Flk-1表达的影响。方法制备大鼠角膜碱烧伤模型，碱烧伤后立即球结膜下注射Es（1mg／ml）0．1ml，隔天一次，用等量生理盐水做对照。实验过程中观察大鼠CNV生长情况。免疫组织化学（IHC）检测Flk一1表达。结果（1）正常大鼠角膜未见Flk-1表达。碱烧伤使角膜Flk-1蛋白表达明显增强，B组与A组比较差异有统计学意义（P〈0．01）。（2）C组Flk-1表达被显著抑制，与B组比较差异有统计学意义（P〈O．01）。（3）C组CNV平均生长长度和速度较B组显著被抑制，各时间点两组比较差异有统计学意义（P〈0．01）。结论（1）碱烧伤后角膜内Flk-1表达显著增加，Flk—1表达增加是碱烧伤后促进CNV形成的重要原因。（2）ES通过抑制Flk—1的表达阻断血管内皮生长因子VEGF信号传导途径，从而抑制了CNV的形成。Objective To investigate the effect of ES on inhibiting CNV and the expression of Flk-1. Method ES and normal saline were separately injected into subconjunctival tissues immediate- ly after alkali-burning,and the manipulation was repeated every other day. The expression of Flk-1 was examined by immunohistochemical method. The length and growth velocity of the newly formed corneal vessels were also measured. Results 1.Flk-1 was not expressed in the cornea. When the cornea was burned by alkali,the expression was dramatically increased. There was a signif- icant difference between group B and group A （P 〈0.01）. 2.The expression of Flk-1 was significant- ly inhibited by ES. There was a significant difference between the group C and group B （P 〈0.01）. 3.The CNV in the group C formed later and grew more slowly than that of group B （P 〈0.01）. Conclusions 1.After alkali-burning, the expression of Flk-1 is greatly enhanced. The increase of Flk-1 is the very important causation of the CNV. 2.ES can significantly inhibit the expression of Flk-1, ES can greatly decrease the bioactivity of VEGF by inhibiting the expression of Flk-l. So it can significantly inhibit the forming of CNV.

关 键 词：内皮抑素 FLK-1 CNV 碱烧伤 

分 类 号：R772.2[医药卫生—眼科]