蛋白酶激活受体-2在急性坏死性胰腺炎大鼠肠黏膜组织中的表达  

Expression of protease activated receptors-2 in intestinal mucosa of acute necrotizing pancreatitis in rats

作　　者：夏亮[1] 陈江[2] 刘丕[2] 苏涛[2] 胡滢[2] 吕农华[2] 

机构地区：[1]南昌大学第一附属医院重症医学科,330006 [2]南昌大学第一附属医院消化内科,330006

出　　处：《中华消化杂志》2012年第9期598-601,共4页Chinese Journal of Digestion

基　　金：江西省青年科学基金（20114BAB215050）

摘　　要：目的探讨蛋白酶激活受体-2（PAR-2）的表达与急性坏死性胰腺炎（ANP）大鼠肠黏膜屏障损伤的相关性。方法制备ANP大鼠模型，采用免疫组织化学法、Western印迹法及RT—PCR方法检测假手术对照组和造模后6、12、24h大鼠肠黏膜组织中PAR-2的表达。组间数据比较采用单因素方差分析。结果免疫组织化学法显示，假手术对照组大鼠小肠黏膜组织中PAR-2的表达较微弱。ANP造模后，PAR～2阳性细胞表达数量明显增加，染色强度明显增强。造模后6、12、24h的免疫组织化学评分分别为4．88±0．33、5．87±0．32、11．17±0．27，与假手术对照组（2．86±0．31）相比，差异有统计学意义（F=747．08，P〈0．01）。假手术对照组大鼠小肠黏膜中PAR-2的mRNA和蛋白表达量均较少，随着ANP造模时间的延长，两者的表达水平均逐渐升高，造模后6、12、24h，mRNA分别为0．56土0．03、0．69±0．03、1．05±0．05，蛋白分别为0．28±0．02、0．35±0．03、0．69±0．04；各时间点与假手术对照组相比，差异均有统计学意义（F=785．69、1177．82，P值均〈O．01）。结论PAR-2在ANP炎性反应中被激活，在肠黏膜屏障损伤的发生发展过程中发挥重要作用。Objective To explore the correlation between the expression of protease activated receptors-2 （PAR-2） and intestinal mucosal barrier injury of acute necrotizing panereatitis （ANP） in rats. Methods The ANP rat model was created. The expression of PAR-2 in rat＇s intestinal mucosa of sham-operated group and ANP group at six, 12 and 24 hours after model established was detected by immunohistochemistry （IHC）, reverse transcription-polymerase chain reaction （RT-PCR） and Western blot. The difference between groups was analyzed by one way analysis o{ variance. Results The results of IHC indicated that PAR 2 expression in rat＇s intestinal mucosa of sham-operated group was weak. The number of PAR-2 expression positive cells and immunostaining intensity increased significantly after ANP model established. The IHC score was 4.88±0.33, 5.87±0.32 and 11.17±0.27 at six, 12 and 24 hours after model established respectively. Compared with those of sham- operated group （2.86 ± 0.31 ）, the differences were statistically significant （F = 747.08, P〈 0.01 ）. The expression of PAR-2 at mRNA and protein level in intestinal mucosa of sham-operated group was very low. As time extended after ANP model established, both expression increased gradually. The PAR-2 mRNA was 0. 56±0. 03, 0. 69±0. 03, 1.05±0.05, and the protein was 0. 28±0. 02, 0.35±0.03, 0.69±0.04 at six, 12 and 24 hours after model established respectively. Compared with shamoperated group, the differences were statistically significant at each time point （F=785.69, 1177.82, both P〈0.01）. Conclusions PAR-2 is activated in the inflammatory progress of ANP, and may play an important role in the pathogenesis and development of intestinal mueosa barrier iniury in ANP.

关 键 词：胰腺炎 急性坏死性 肠黏膜 受体 蛋白酶激活 疾病模型 动物 

分 类 号：R576[医药卫生—消化系统]