β-arrestinl在胃癌细胞BGC-823中的生物学功能  

Biological role of β-arrestinl in human gastric cancer BGC-823 cells

作　　者：王旭[1] 王璐[1] 董菁[1] 巴亚斯古楞[1] 任建林[1] 

机构地区：[1]厦门大学附属中山医院消化科,361004

出　　处：《中华消化杂志》2012年第9期615-619,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金（30971362）;福建省青年科技人才创新项目（2006F3127）

摘　　要：目的探讨β-arrestinl对胃癌细胞BGC-823增殖、迁移、侵袭及凋亡能力的影响。方法用实时定量PCR技术及Western印迹法检测人胃黏膜上皮细胞GES和人胃癌细胞株BGC-823、MKN-28、SGC-7901中parrestinl的表达水平。应用RNA干扰技术构建稳定干扰β-arrestinl和阴性对照组（pU6空载体）的BGC-823细胞株。进一步应用细胞计数法、划痕实验、Transwell小室实验及流式细胞术分析干扰β-arrestinl和阴性对照组的BGC-823细胞株的增殖、迁移、侵袭能力及凋亡水平的变化。统计学处理采用t检验。结果β-arrestinl在GES表达量为0．001士0．001，MKN-28表达量为0．002±0．000，SGC-7901表达量为0．003±0．002，BGC-823中表达量为0．005±0．000。干扰3-arrestinl和阴性对照的BGC-823细胞增殖抑制率分别为-30．2％和100．0％，迁移能力受到抑制，穿过基质膜细胞数分别为126．25±3．24和213．50±6．27（t=0．000，P〈O．01），凋亡率为（41．350±1．053）％和（11．497±0．589）％（t=0．015，P〈0．05）。结论β—arrestinl在胃癌细胞中高水平表达，并且随着胃癌细胞恶性度增高而表达量增加，在BGC-823细胞中干扰parrestinl后能抑制细胞的生长、迁移、侵袭，并提高凋亡水平。Objective To investigate the effects of β-arrestinl on proliferation, migration, invasion and apoptosis of human gastric cancer BGC-823 cell line. Methods The expression of β- arrestinl in human gastric epithelial cell line GES, human gastric cancer cell line BGC-823, MKN-28 and SGC-7901 was detected by realtime-polymerase chain reaction （PCR） and Western blot. The stable β-arrestinl and negative control interfered BGC-823 cell line were established by RNA interference technology. The cell proliferation, migration, invasion and cell apoptosis of β-arrestinl stable interfered BGC-823 cell line was examined by cell counting, scratch test, Transwell chamber test and flow cytometry assays. The data were analyzed by t test. Results The expression of β- arrestinl in cell line GES, MKN-28, SGC-7901 and BGC-823 was 0. 001 ± 0. 001, 0. 002±0. 000, 0. 003± 0. 002 and 0. 005 ± 0. 000 respectively. The inhibition ratio of proliferation in β-arrestinl interfered BGC-823 cells and negative control cells were -30.2% and 100.0%. The invasion ability was also inhibited, the number of migratory cells was 126. 25±3.24 and 213. 50±6.27 （t=0. 000, P〈0.01）, and the apoptosis rate was （41. 350±1. 053）% and （11. 497±0. 589）% （t=0. 015, P〈0.05）. Conclusions β-arrestinl is highly expressed in gastric carcinoma, and the expression increased along with the malignancy degree. The cell proliferation, migration and invasion is inhibited by interference of β-arrestinl in BGC-823 cells, while the cell apoptosis is promoted.

关 键 词：β-arrestin1 胃肿瘤 RNA干扰 肿瘤细胞 培养的 

分 类 号：R735.2[医药卫生—肿瘤]