温郁金提取物对食管癌TE-1细胞增殖的抑制作用  

The Molecular Mechanisms of Curcuma Wenyujin Extract-mediated Inhibitory Effects on Human Esophageal Carcinoma Cells in Vitro

作　　者：景钊[1] 邹海洲[2] 许芳[3] 邹长林[1] 

机构地区：[1]温州医学院附属第一医院放化疗科,浙江温州325000 [2]温州医学院附属第一医院中医科,浙江温州325000 [3]温州医学院生命科学学院,浙江温州325000

出　　处：《中国中西医结合杂志》2012年第9期1219-1222,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省中医药资助项目(No.2008CB051)

摘　　要：目的研究温郁金提取物对食管癌TE-1细胞增殖的抑制作用。方法采用超临界CO2萃取法提取温郁金成分。将TE-1细胞贴壁后分成4组:1组加入100μL含0.1%DMSO的RMPI-1640培养基,作为对照组;另外3组分别加入浓度为25、50、100mg/L温郁金提取物完全培养液各100μL,分别作为温郁金低、中、高剂量组。培养48h后采用四甲基偶氮唑盐(MTT)比色法测定各组TE-1细胞生长抑制率。应用基因芯片技术检测各组TE-1细胞基因表达谱的变化。对差异表达基因进行基因本体(gene ontology,GO)功能分类。结果与对照组比较,温郁金各剂量组生长抑制率均升高,差异有统计学意义(P<0.05)。温郁金各剂量组TE-1细胞生长抑制率随着剂量升高而增加,差异均有统计学意义(P<0.05)。通过基因芯片筛选出温郁金提取物作用前后88个基因表达水平发生改变且有统计学意义,包括22个基因表达下调,66个基因表达上调。基因GO分类提示差异表达基因主要涉及信号传导(6个)、细胞周期(8个)、凋亡(14个)或分化调节(10个)等。结论温郁金提取物对人食管癌TE-1细胞生长有抑制作用,其抗肿瘤作用可能与调控多层次的基因表达改变有关。Objective To study the molecular mechanisms of Curcuma Wenyujin extract-mediated inhibi- tory effects on human esophageal carcinoma cells. Methods The Curcuma Wenyujin extract was obtained by su- percritical carbon dioxide extraction. TE-1 cells were divided into 4 groups after adherence. 100 IJL RMPI-1640 culture medium containing 0. 1% DMSO was added in Group 1 as the control group, 100 uL 25, 50, and 100 mg/L Curcuma Wenyujin extract complete culture medium was respectively added in the rest 3 groups as the low, middle, and high dose Curcuma Wenyujin extract groups. The effects of different doses of Curcuma Wenyu- jin extract （25, 50, and 100 mg/L）on the proliferation of human esophageal carcinoma cell line TE-1 in vitro were analyzed by MIF assay. The gene expression profile was identified by cDNA microarrays in esophageal carcino- ma TE-1 cells exposed to Curcuma Wenyujin extract for 48 h. The differential expression genes were further ana- lyzed by Gene Ontology function analysis. Results Compared with the control group, MTT results showed that Curcuma Wenyujin extract significantly inhibited the proliferation of TE-1 cells in a dose-dependent manner （ P 〈 0. 05）. The expression level of 88 genes changed with significance, including 66 up-regulation genes and 22 down-regulation genes. Gene Ontology analysis indicated the genes coding for proteins was involved in signal transduction （6）, cell cycle （8） ,apoptosis （14）, and cell differentiation （10）. Conclusions The Curcuma We- nyujin extract could inhibit the growth of human esophageal carcinoma cell line TE-1 in vitro. The molecular mech- anisms might be associated with regulating genes expressions at multi-levels.

关 键 词：温郁金 食管癌 TE-1细胞 基因芯片 

分 类 号：R735.1[医药卫生—肿瘤] R285.5[医药卫生—临床医学]