microRNA-10b表达变化对人胰腺癌细胞生物学行为的影响  

Effect of microRNA-10b expression alteration on biological behavior of human pancreatic cancer cells

作　　者：向春晖 郑岩松[2] 石铮[2] 

机构地区：[1]湖北省恩施自治州中心医院外三科,湖北恩施445000 [2]福建医科大学附属第一医院肝胆胰外科,福建福州350005

出　　处：《中国普通外科杂志》2012年第9期1091-1096,共6页China Journal of General Surgery

基　　金：福建省自然科学基金资助项目(2009J01134)

摘　　要：目的：探讨microRNA-10b（miR-10b）的表达变化对人胰腺癌细胞ASPC-1生物学行为的影响。方法：胰腺癌细胞ASPC-1细胞株分别以脂质体法转染正义、反义miR-10b真核表达载体和空载体,用荧光显微镜观察转染效率。以未转染的ASPC-1细胞为空白对照,检测各转染组miR-10b和RhoC蛋白表达,并检测各组细胞生长、凋亡及侵袭能力。结果：各组转染48 h后,转染效率达50%~70%;与空白对照组比较,正义miR-10b转染组细胞miR-10b表达和RhoC蛋白表达量明显增加,凋亡率明显降低,生长活性和侵袭能力明显增强（均P〈0.05）;而反义miR-10b转染组细胞以上检测结果与正义miR-10b转染组细胞呈相反改变（均P〈0.05）;空载体转染组所有指标与空白对照组间无明显差异（均P〉0.05）。结论：上调miR-10b的表达,能促进胰腺癌细胞ASPC-1的生长、侵袭并抑制凋亡,该作用可能与其调控RhoC表达有关。Objective： To investigate the effect of microRNA-10b（miR-10b） expression alteration on the biological behavior of human pancreatic cancer ASPC-1 cells. Methods： ASPC-1 cells were transfected with the eukaryotic expression plasmid vector containing pre-miR-10b and antisense miR-10b or empty vector by lipofectamine,respectively,and the transfection efficiency was measured with fluorescence microscopy.Using the non-transfected ASPC-1 cells as blank control,the miR-10b and RhoC protein expression of the transfected cells in each group were detected,and their growth,apoptosis and invasve ability were also examined. Results： The transfection efficiencies of each group were about 50%-70% 48 h after transfection.By comparison with blank control group,cells in pre-miR-10b transfection group showed increased expression of miR-10b and RhoC protein,decreased apoptosis,and enhanced proliferative and invasion ability（all P〈0.05）,while the results of above indexes in antisense miR-10b transfection group showed the opposite changes to those in pre-miR-10b transfection group（all P〈0.05）.Bat there were no significant differences in all determined parameters between empty vector transfection group and blank control group（all P〈0.05）. Conclusion： Up-regulation of miR-10b in ASPC-1 cells can promote their growth and invasive ability with reduced apoptosis,and these effects may probably be associated with its action on regulation of RhoC expression.

关 键 词：胰腺肿瘤 MicroRNA-10b 转染 细胞增殖 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]