牛磺酸对反复力竭运动大鼠骨骼肌线粒体的保护作用  

Effect of taurine on skeletal muscle mitochondria in rats subjected to repeated exhausting exercises

作　　者：库建斌[1] 余斌[2] 覃承诃[2] 罗吉伟[2] 

机构地区：[1]解放军第四二一医院骨科,广州510000 [2]南方医科大学南方医院创伤骨科

出　　处：《中华创伤骨科杂志》2012年第9期792-794,共3页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金（30801162）

摘　　要：目的探讨牛磺酸对反复力竭运动大鼠骨骼肌线粒体的保护作用。方法雄性SD大鼠30只随机分为安静对照组、力竭对照组、牛磺酸组，每组10只。安静对照组和力竭对照组大鼠每天按0．8mL／kg灌喂生理盐水1次；牛磺酸组大鼠每天用200mg／L牛磺酸按200mg／kg灌喂1次。力竭对照组和牛磺酸组大鼠运动前先连续灌喂15d，力竭运动期间（30d）每天继续灌喂。安静对照组大鼠整个实验过程中不运动，只灌喂生理盐水，灌喂时间与力竭对照组和牛磺酸组一样，共45d。运动后，处死大鼠，切取股四头肌，并对大鼠体质量及股四头肌湿重进行比较；行透射电镜观察，着重观察股四头肌线粒体形态。结果力竭对照组体质量、股四头肌湿重显著低于安静对照组和牛磺酸组，力竭对照组大鼠骨骼肌肌丝排列紊乱，线粒体肿胀和空泡变性，牛磺酸组大部分骨骼肌超微结构图接近正常。结论口服牛磺酸对反复力竭运动大鼠骨骼肌线粒体具有保护作用，它减少反复力竭收缩骨骼肌的坏死丢失。Objective To investigate the protective effect of taufine on the skeletal muscle mito- chondria in rats subjected to repeated exhausting exercises on a treadmill. Methods Thirty SD male rats were randomized into a quiet control （QC） group （ n = 10）, an exhausting exercise control （EEC） group （ n = 10） and an exhausting exercise plus taurine treatment （TT） group （ n = 10）. QC and EEC groups were fed with normal saline （dose： 0.8 mL/kg） once per day; TT group was fed with taurine （concentration： 200 mg/L; dose： 200 mg/kg） once per day. The EEC and TT groups were fed continuously for 15 days pre-exercise and 30 days during the exhausting exercises. The rats in the QC group were subjected to no exercise other than the feeding for 45 days as in the other 2 groups. After repeated exhausting running on a treadmill in a course of 30 days, all the rats were sacrificed to harvest the quadriceps femoris muscles. The body mass of the rats and the net mass of the quadrieeps femoris were measured. Transmission electron microscopy was used to observe the morphology of the mitochondria. Results The body mass of the rats and the net mass of the quadriceps femoris were obviously lower in the EEC group than in the other 2 groups. The myofilaments in the quadriceps femoris were deranged, the mitochondria swelling and the vacuole degenerated in the EEC group. The ultra-structures of the quadriceps femoris and mitochondria were roughly normal in the TT group as in the QC group. Conclusion Oral taurine administration can protect and improve the mitochondrial function in the skeletal muscles in rats taking repeated exhausting exercises, reducing necrosis and loss of the skeletal muscles caused by the exhausting exercises.

关 键 词：肌 骨骼 线粒体 超微结构 牛磺酸 力竭运动 

分 类 号：R285.5[医药卫生—中药学]