尿多酸肽诱导急性早幼粒细胞白血病细胞株凋亡的实验研究  

Uroacitides induce apoptosis of acute promyelocytic leukemia cells in vitro

作　　者：周宏[1] 万海英[1] 李冬[1] 

机构地区：[1]同济大学附属同济医院检验科,上海200065

出　　处：《同济大学学报（医学版）》2012年第4期13-18,共6页Journal of Tongji University(Medical Science)

摘　　要：目的探讨尿多酸肽(CDA-Ⅱ)对急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)细胞NB4及耐维甲酸的NB4-R1细胞的凋亡作用。方法以NB4和NB4-R1细胞作为体外模型,观察CDA-Ⅱ作用前后细胞的形态和生长增殖情况。流式细胞术检测细胞凋亡特征。Western印迹法检测凋亡调控蛋白蛋白激酶C(PKCδ)及Caspase-3的水解活化。结果 CDA-Ⅱ作用于NB4和NB4-R1细胞后,细胞生长受到抑制;形态上观察到分化和凋亡现象;Annexin V-PI法发现,用药24 h后NB4和NB4-R1细胞凋亡比例分别上升至(58.7±3.1)%和(87.8±0.5)%;PKCδ和Caspase-3水解活化。结论 CDA-Ⅱ作用于细胞后,水解活化Caspase-3,裂解PKCδ生成活性片段,诱导细胞凋亡。Objective To investigate the apoptosis-inducing effect of Uroacitides（ CDA- Ⅱ ） on acute promyelocytic leukemia （APL） cell line NB4 and NB4-R1 （retinoic acid resistant）. Methods The cultured NB4 and NB4-R1 cells were treated with different concentrations of CDA-Ⅱ. The changes of cell morphology were observed; cell proliferation and growth inhibition was measured by cell counting; apoptosis was examined by flow cytometry; the activation of protein kinase C （PKCδ） and Caspase-3 was detected by Western blotting. Results After treated by CDA- Ⅱ the growth of NB4 and NB4-R1 cells was inhibited; morphological differentiation and apoptosis were observed. Annexin V-PI method showed that cell apoptosis rates were increased to （58.7± 3.1 ） % and （87.8 ± 0.5 ） % for NB4 and NB4-R1 cells respectively after 24 h of treatment, and PKC8 and Caspase-3 were activated. Conclusion CDA- Ⅱ can induce apoptosis of NB4 and NB4-R1 cells by activating the protein Caspase- 3 and PKCδ.

关 键 词：尿多酸肽 急性早幼粒细胞白血病 细胞凋亡 维甲酸耐药 

分 类 号：R733.7[医药卫生—肿瘤]