Chromophore-assisted laser inactivation in neural development  

作　　者：Wei Li Nico Stuurman Guangshuo Ou 

机构地区：[1]National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China [2]The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University ofCalifornia, San Francisco, San Francisco, CA 94158, USA

出　　处：《Neuroscience Bulletin》2012年第4期333-341,共9页神经科学通报（英文版）

基　　金：supported by grants from the National Basic Research Program of China (973 Program) to W.L.and G.O.(2012CB966800 and 2012CB945002);the National Natural Science Foundation of China to W.L.and G.O.(31101002,31171295 and 31190063);the Junior Thousand Talents Program of China to G.O

摘　　要：Chromophore-assisted laser inactivation（CALI） is a technique that uses photochemically-generated reactive oxygen species to acutely inactivate target proteins in living cells.Neural development includes highly dynamic cellular processes such as asymmetric cell division,migration,axon and dendrite outgrowth and synaptogenesis.Although many key molecules of neural development have been identified since the past decades,their spatiotemporal contributions to these cellular events are not well understood.CALI provides an appealing tool for elucidating the precise functions of these molecules during neural development.In this review,we summarize the principles of CALI,a recent microscopic setup to perform CALI experiments,and the application of CALI to the study of growth-cone motility and neuroblast asymmetric division.Chromophore-assisted laser inactivation（CALI） is a technique that uses photochemically-generated reactive oxygen species to acutely inactivate target proteins in living cells.Neural development includes highly dynamic cellular processes such as asymmetric cell division,migration,axon and dendrite outgrowth and synaptogenesis.Although many key molecules of neural development have been identified since the past decades,their spatiotemporal contributions to these cellular events are not well understood.CALI provides an appealing tool for elucidating the precise functions of these molecules during neural development.In this review,we summarize the principles of CALI,a recent microscopic setup to perform CALI experiments,and the application of CALI to the study of growth-cone motility and neuroblast asymmetric division.

关 键 词：chromophore-assisted laser inactivation growth cone NEUROBLAST asymmetric cell division 

分 类 号：Q421[生物学—神经生物学] O634.41[生物学—生理学]