苦豆子多糖硫酸化修饰工艺的研究  

Sulfation of polysaccharides in Sophora alopecuroides L.

作　　者：赵永安[1,2] 郑晓辉[1,2] 张岩[3,2] 陶遵威[2] 

机构地区：[1]天津医科大学,天津300070 [2]天津市医药科学研究所,天津300020 [3]天津中医药大学,天津300073

出　　处：《中南药学》2012年第8期580-583,共4页Central South Pharmacy

基　　金：天津市自然科学基金(No.10JCZDJC21000)

摘　　要：目的确定氨基磺酸-甲酰胺法修饰苦豆子多糖的可行性,并应用正交试验法对苦豆子多糖硫酸化修饰工艺进行优化。方法以硫酸根取代度为指标,采用L9(34)正交试验设计对氨基磺酸用量、甲酰胺用量、反应时间、反应温度进行优选。结果苦豆子多糖硫酸化修饰过程中,取苦豆子多糖30mg在氨基磺酸135mg,反应温度100℃,反应时间4h,甲酰胺20mL的工艺条件下,苦豆子多糖可获得理想的硫酸根取代度,通过紫外光谱和红外光谱检测,苦豆子多糖的糖基单元已成功引入硫酸基团。结论氨基磺酸-甲酰胺法适用于苦豆子多糖的硫酸化修饰,此方法有效可行,具有较好的硫酸化修饰效果。Objective To confirm the feasibility of sulphamic acid-formamide method and to optimize the sulfated modification conditions of Sophora alopecuroides polysaccharide（SAP）.Methods SAP was modified by sulphamic acid-formamide.L9（34） orthogonal test was applied to optimize the sulphamic acid dosage,formamide volume,reaction time,and reaction temperature with the degree of the sulfate group as the index.Results The optimized modification conditions were sulphamic acid 135 mg,reaction temperature 100 ℃,reaction time 4 h,and formamide 20 mL.Using the optimized modification conditions,we obtained the best sulfated Sophora alopecuroides polysaccharide（SAPS）.UV and infrared spectrum demonstrated the modification was successful.Conclusion The sulphamic acid-formamide method is effective and feasible to modify SAP.

关 键 词：苦豆子多糖 硫酸化修饰 氨基磺酸-甲酰胺法 正交试验 

分 类 号：R914[医药卫生—药物化学]