利用曲霉sp. HX-1发酵稻草秸秆制备纤维素酶  

Production of Cellulase by Solid Fermentation of Straw Stalk Using Aspergillus sp.

作　　者：刘彪[1] 马娟[1] 谭玲[1] 林永慧[1] 王海燕[1] 刘任成[1] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2012年第4期95-100,共6页Journal of Jishou University(Natural Sciences Edition)

基　　金：吉首大学大学生研究性学习和创新性实验计划项目(JSU-CX-2010-42);吉首大学植物资源与利用重点实验室开放基金资助项目(JSK201109);湖南省教育厅科学研究资助项目(09C788;10C1118);吉首大学人才引进启动基金资助项目(jsdxkyzz2005);吉首大学科技处科研资助项目(10JD028)

摘　　要：以稻草秸秆原料,利用曲霉sp. HX-1固态发酵生产纤维素酶,研究了不同发酵时间、不同氮源和氮源浓度对曲霉sp. HX-1纤维素酶系的影响,并最终用硫酸铵分级沉淀和低温冷冻干燥的方法得到混合纤维素酶干品.结果表明,发酵6d后稻草秸秆产生的纤维素酶活力单位最大,分别为CMC酶307U/mL,C1酶841U/mL、β-葡萄糖苷酶205U/mL.以不同氮源优化发酵条件时发现,NH4NO3质量浓度为1g/L时CMC酶活力达到1 652 U/mL,且失重率也到达最大值17.42%;NH4Cl质量浓度为0.5g/L时,C1酶活力达到1 807U/mL;尿素(UREA)质量浓度为2.0g/L时,β-葡萄糖苷酶活力为2 033U/mL,这表明氮源对曲霉sp.HX-1纤维素酶系的影响很大.最后在NH4NO3质量浓度为1.0g/L的条件下,将120g稻草秸秆发酵6d,从发酵液中提取得到8.851 7g混合纤维素酶的干品.此实验为以后探讨碳源或者其他因素的影响提供方法借鉴,也可以为获得纤维素酶混合酶干品的获得提供参考方法.This paper expounds the experiment on cellulase solid fermentation under the conditions of dif- ferent fermentation days, nitrogens sources and nitrogen concentration. The experiment used the straw stalk as raw substrate,and finally obtained dry product which was high in cellulase by methods of ammo- nium sulfate fractionation and freeze-drying. The researches demonstrated that cellulase activity of en- doglucanase, exoglucanase and 13-glucosidase could reach 1652,841,205 U/mL, respectively, after 6 days＇ fermentation. The optimal nitrogen sources and nitrogen concentrations to generate cellulase by Aspergil- lus sp. were also investigated. The results also showed that endoglucanase, exoglucanase and 13-glucosi- dase could reach 1652 U/mL（NH4NO3 1.0 g/L）,l 807 U/mL（NH4C1 0.5 g/L）,2033 U/mL（UREA 2.0 g/L） ,respectively. What＇s more,the weight loss ratio reached 17.42~ which is the maximum value under the optimal endoglucanase fermentation condition. It also demonstrated that nitrogen sources have a great impact on cellulose production. At last,8. 8517 g dry product was obtained from 120 g straw fermentation broth which fermented under optimal nitrogen source （NH4 NO3 ）, nitrogen concentration （1.0 g/L） and fermentation days （6 d）.

关 键 词：曲霉sp. HX-1 稻草秸秆 纤维素酶 氮源 硫酸铵分级沉淀 低温冷冻干燥 

分 类 号：TK6[动力工程及工程热物理—生物能]