运用巢式PCR方法进行无创性产前诊断的研究  

Study on the feasibilit of the nested PCR method ovpplied in non-invastive prenatal diagnosis

作　　者：黄睿[1] 兰永连[1] 周丽颖[1] 梁毓[1] 吕巍[1] 贾兴元[2] 

机构地区：[1]首都医科大学附属北京妇产医院生殖医学科,100026 [2]首都医科大学附属北京朝阳医院实验研究中心,100020

出　　处：《中国优生与遗传杂志》2012年第9期9-10,共2页Chinese Journal of Birth Health & Heredity

基　　金：首都医学发展科研基金(项目编号:2009-3130)

摘　　要：目的探讨巢式PCR方法应用于胎儿无创性产前诊断的可行性。方法巢式PCR扩增SRY基因。结果实验共检查62例孕妇,阳性结果 29例,阴性结果 33例。与羊水细胞核型结果对照,特异度100%,灵敏度96.67%。结论运用巢式PCR方法进行无创性产前诊断准确度高,具有可行性,并能为产前无创伤性诊断胎儿常见非整倍体打下基础。Objective：To study the feasibility of the nested PCR method applied in non-invasive prenatal diagnosis.Methods：The nested PCR was performed for the SRY-gene.Results：A total of 62 women were tested,the positive predictive results were 29 case and the negative being 33 cases.Results were compared with the karyotypes of amniotic fluid cell,Sensitivity and specificity of the experiment were 100% and 96.67% respectively.Conclusion： The nested PCR method of non-invasive prenatal diagnosis is feasible and is the basis of fetal aneuploidy of prenatal diagnosis.

关 键 词：胎儿游离核酸 无创伤性 产前诊断 巢式PCR 

分 类 号：R714.55[医药卫生—妇产科学]