全自动DNA图像分析在乳腺细针穿刺细胞学诊断中的应用  

DNA image cytometry in breast fine needle aspiration cytology

作　　者：王蕊[1] 王珩[1] 杜芸[1] 

机构地区：[1]河北医科大学第四医院癌检中心,石家庄050011

出　　处：《肿瘤研究与临床》2012年第8期521-523,共3页Cancer Research and Clinic

摘　　要：目的探讨全自动DNA图像分析（DNA-ICM）系统在乳腺细针穿刺细胞学（FNAC）诊断中的应用价值。方法对95例乳腺肿块患者行细针穿刺，对所获细胞标本分别行常规细胞学诊断及DNA—ICM检测，比较两种诊断方法与组织病理学诊断结果的符合率。结果以组织病理诊断为标准，DNA—ICM与常规细胞学诊断符合率差异无统计学意义[88．4％（84／95）比94．7％（90／95）1（χ^2=2．457，P=0．117）；DNA—ICM检测排除了常规细胞学诊断的2例假阳性（可疑癌细胞）。DNA-ICM检测中，浸润性导管癌异常DNA含量的检出率高于浸润性小叶癌[90．3％（65／72）比50．0％（4／8）]（Fisher精确概率法，P=0．011）。结论DNA—ICM可以降低FNAC的假阳性率，但其对二倍体肿瘤诊断存在局限性。Objective To discuss the diagnosis value of DNA imaging cytometry （DNA-ICM） analysis in fine needle aspiration cytology （FNAC）. Methods Conventional cytopathology and DNA-ICM were used to detect 95 fine needle aspiration patients with breast masses. The results were analyzed according to the histopathological diagnosis. Results There was not significant difference between conventional cytopathology and DNA-ICM [88.4 %（84/95） vs. 94.7 %（90/95）] （χ2 = 2.457,P = 0.117）. Two false positive cases had been found in conventional cytopathologic diagnosis, but not in DNA-ICM. There was a positive correlation between the nucleus area and its DNA content （r = 0.744, P = 0.000）. By DNA-ICM, the detectable rate of abnormai DNA content in the infiltrating ductal carcinoma was higher than that in the infiltrating lobular carcinoma [90.3 %（65/72） vs. 50.0 %（4/8）] （P = 0.011）. Conclusion The detection of DNA-ICM can reduce the false positive rate of FNAC, but it has limitations in diploid tumors.

关 键 词：DNA 图像分析 乳腺肿物 细针穿刺细胞学 

分 类 号：R737.9[医药卫生—肿瘤]