大鼠髁状突颈部骨折对软骨细胞上游刺激因子1表达影响研究  

Effect of unilateral condylar neck fracture on USF1 expression of the chondrocyte in growing rat

作　　者：钱德云 徐自祥 陈建中[2] 王维琦[2] 赵泓霖 

机构地区：[1]云南陆良县人民医院口腔科,云南陆良655600 [2]昆明医科大学口腔医学院,昆明650031 [3]云南省边防总队医院口腔科,昆明650000

出　　处：《中国实用口腔科杂志》2012年第8期473-475,共3页Chinese Journal of Practical Stomatology

基　　金：云南省教育厅基金项目(2011Y198)

摘　　要：目的研究发育期SD大鼠髁状突颈部骨折对下颌髁状突软骨细胞中上游刺激因子1(USF1)表达的影响。方法本研究于2011年7—9月在昆明医科大学口腔医学院研究所完成。选择4周龄雄性SD大鼠12只,其中6只大鼠用于制作下颌单侧髁状突颈部骨折动物模型,6只大鼠为对照。分别在术后1、3、5周时处死大鼠,取出骨折侧、骨折对侧以及空白对照的髁状突软骨。采用免疫组织化学方法检测髁状突软骨细胞中USF1表达情况。结果术后1周,骨折对侧空白对照组与骨折侧的USF1表达有显著性差异(P<0.01);术后3周,空白对照组与骨折侧USF1表达有显著性差异(P<0.01)。骨折侧术后3周、5周与术后1周USF1表达有显著性差异(P<0.01)。结论单侧髁状突颈部骨折引起应力变化,使患侧髁状突软骨细胞中USF1表达异常,进而影响髁状突的生长发育。Objective To investigate the effect of unilateral condylar neck fracture on USF1 expression of the chondrocyte in growing rats. Methods Twelve 4-week-old rats were randomly divided into two parts： half were operated to cause condylar fracture, the other part as control. The rats were killed at 1,3 and 5 weeks after surgery, and the condylar cartilage was obtained from the operating side and non-operating side of experimental group and control group. Immunohistochemical staining was used to qualitively examine USF1 expression. Results There was significant difference between non-operating side, control group and operating side at 1 week after surgery. There was significant difference between operating side and control group at 3 weeks after surgery. There was significant difference between 3 and 5 weeks and 1 week after surgery on operating side. Conclusion The unbalanced stress caused by unilateral condylar neck fracture change USF expression affect the development and growth of condyle.

关 键 词：骨折 髁状突软骨 上游刺激因子1 大鼠 软骨细胞 

分 类 号：R78[医药卫生—口腔医学]