发酵-渗透汽化连续耦合合成ABE的动力学  

Kinetics of ABE Synthesis by Continuous Coupling of Fermentation with Pervaporation

作　　者：张俊青[1] 陈春燕[1] 崔海娣[1] 李伟佳[1] 应超[1] 石尔[2] 肖泽仪[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065 [2]长沙理工大学能源与动力工程学院,湖南长沙410076

出　　处：《精细化工》2012年第9期866-869,共4页Fine Chemicals

基　　金：国家自然科学基金项目(20776088);湖南省教育厅资助项目(08C114);湖南省科技厅资助项目(2008SK4025)~~

摘　　要：采用PDMS膜生物反应器和丙酮丁醇梭菌进行了生产ABE的封闭循环连续发酵实验,研究了发酵和渗透汽化分离连续耦合条件下的发酵动力学行为。发酵-分离连续耦合实验运行持续时间长达192 h。运行过程中,细胞质量浓度维持在0.84～4.00 g/L,发酵液中ABE的总质量浓度为5.14～17.54 g/L,葡萄糖质量浓度大约为16.08～35.15 g/L,总体积产率为0.36 g/(L.h)。结果表明,膜生物反应器系统运行稳定,发酵-渗透汽化分离连续耦合生产ABE的操作模式具有可行性和优越性。Continuous coupling of acetone-butanol-ethanol （ ABE ） fermentation with pervaporation was conducted in a continuous and closed-circulating fermentation system by combining Clostridium acetobutylicum and PDMS membrane bioreactor. The continuous fermentation successfully lasted for 192 h, and the corresponding kinetic behaviors were studied. During the continuous fermentation, the cell mass concentrations were kept within the range of 0. 84 - 4. O0 g/L, the glucose mass concentrations remained within the range of 16.08 N 35.15 g/L, and the total solvents mass concentrations in the fermentation broth were within 5.14 - 17.54 g/L. Under such conditions, ABE productivity was 0. 36 g/（ L · h ）. The results show that ABE fermentation continuously coupling with pervaporation in a PDMS membrane bioreactor system is feasible and promising.

关 键 词：ABE发酵 渗透汽化 动力学 丙酮丁醇梭菌 生物工程 

分 类 号：TQ92[轻工技术与工程—发酵工程]