维生素D缺乏肾精不足小鼠模型的建立  

Model establishment of kidney essence deficiency by vitamin D deficiency in mice

作　　者：陈云志[1] 杨长福[1] 秦钟[1] 张小容[1] 柴艺汇[1] 王和生[1] 

机构地区：[1]贵阳中医学院基础医学院,贵阳550002

出　　处：《中华中医药杂志》2012年第9期2412-2414,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81160473);贵州省科技厅;贵阳中医学院联合基金(No.黔科合中药字[2010]LKZ7017);贵州省科技厅科技基金(No.黔科合J字[2012]2064)~~

摘　　要：目的:由维生素D缺乏小鼠模型性激素改变探讨制作肾精不足模型的可行性。方法:运用干扰维生素D合成的两个途径(避光及饮食),制作维生素D缺乏小鼠模型,同时以氢化可的松制作小鼠肾阴虚、肾阳虚模型,观察比较25(OH)D3、睾酮、雌二醇、黄体生成素变化。结果:与正常对照组比较,VD缺乏组25(OH)D3、睾酮、雌二醇降低(P<0.05);与VD缺乏组比较,肾阴虚组、肾阳虚组25(OH)D3、睾酮、雌二醇升高,但均较正常组降低(P<0.05)。结论:维生素D缺乏小鼠模型性激素改变,可以作为肾精不足研究模型。Objective： To discuss the easibility of establishment of the kidney essence deficiency model by sexual hormone changes induced by vitamin D deficiency. Methods： Interference of vitamin D synthesis of two pathways （obstacle light and diet ）, to establish of vitamin D deficiency model, at the same time use hydrocortisone making kidney yin deficiency, kidney yang deficiency model, compared different group with 25 （OH） D3, testosterone, estradiol and luteinizing hormone. Results： Compared with normal control group, kidney-yang deficiency group and kidney-yin deficiency group, 25 （OH） D3, testosterone and estradiol of vitamin D deficiency mouse model group were decreased （P〈0.05）. Compared with normal control group, luteinizing hormone of vitamin D deficiency mouse model group was decreased （P〈0.05）. Conclusion： Vitamin D deficiency deficiency mouse model has sex hormone decrease, it can be used as the deficiency of the kidney essence research model.

关 键 词：肾精不足 模型 维生素D 睾酮 雌二醇 

分 类 号：R-332[医药卫生]