Cep57, a NEDDl-binding pericentriolar material component, is essential for spindle pole integrity  

作　　者：Qixi Wu Runsheng He Haining Zhou Albert CH Yu Bo Zhang Junlin Teng Jianguo Chen 

机构地区：[1]The State Key Laboratory of Biomembrane and Membrane Bioengineering and The Key Laboratory of Cell Proliferation and Dif ferentiation of Ministry of Education,College of Life Sciences,Peking University,Beijing 100871,China [2]Department of Neurobiology,Neuroscience Research Institute,School of Basic Medical Sciences,Peking University,Beo'ing 100191,China [3]The Center for Theoretical Biology,Peking University,Beijing 100871,China

出　　处：《Cell Research》2012年第9期1390-1401,共12页细胞研究（英文版）

基　　金：We thank Chuanmao Zhang, Xiaoyan Zhang and Jingyan Fu for TPX-2 antibody and NEDD1 plasmids; Masatoshi Takeichi and Wenxiang Meng for the ct-tubulin-pCAsalGFP plasmid. This work was supported by the National Natural Science Foundation of China （30971433, 31171283） and the National Basic Research Program of China （973 Program; 2010CB833705）.

摘　　要：Formation of a bipolar spindle is indispensable for faithful chromosome segregation and cell division. Spindle integrity is largely dependent on the centrosome and the microtubule network. Centrosome protein Cep57 can bundle microtubules in mammalian cells. Its related protein （Cep57R） in Xenopus was characterized as a stabilization factor for microtubule-kinetochore attachment. Here we show that Cep57 is a pericentriolar material （PCM） component. Its interaction with NEDD1 is necessary for the centrosome localization of Cep57. Depletion of Cep57 leads to unaligned chromosomes and a multipolar spindle, which is induced by PCM fragmentation. In the absence of Cep57, centrosome microtubule array assembly activity is weakened, and the spindle length and microtubule density decrease. As a spindle microtubule-binding protein, Cep57 is also responsible for the proper organization of the spindle microtubule and localization of spindle pole focusing proteins. Collectively, these results suggest that Cep57, as a NEDD1- binding centrosome component, could function as a spindle poleand microtubule-stabilizing factor for establishing robust spindle architecture.

关 键 词：Cep57 CENTROSOME SPINDLE cell cycle 

分 类 号：Q253[生物学—细胞生物学] TB301[一般工业技术—材料科学与工程]