TGFBI对胃癌细胞SGC-7901转移潜能影响的研究  

TGFBI induces SGC-7901 adhesion,proliferation and migration

作　　者：李震[1] 苗志峰[1] 孙公平[2] 沈名扬[2] 张家魁[2] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤科,辽宁沈阳110001 [2]中国医科大学附属第四医院胃肠外科,辽宁沈阳110032

出　　处：《中华肿瘤防治杂志》2012年第15期1121-1123,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81071956)

摘　　要：目的:探讨细胞外基质蛋白转化生长因子β诱导(TGFBI)对胃癌细胞SGC-7901转移潜能的影响。方法:牛血清白蛋白(BSA)、纤维粘连蛋白(FN)及细胞外基质蛋白TGFBI包被96孔板后,于其上培养SGC-7901细胞。MTT法检测其与TGFBI粘附能力及粘附后的增殖能力。同法包被Transwell小室滤膜下表面,检测其诱导SGC-7901细胞迁移的能力。结果:FN和TGFBI支持SGC-7901细胞粘附的能力明显高于BSA(P=0.023),FN和TGFBI之间差异无统计学意义,P>0.05;BSA、FN和TGFBI诱导SGC-7901细胞增殖的能力渐次增强,P=0.031;FN和TGFBI诱导SGC-7901细胞迁移的能力明显高于BSA(P=0.019),FN和TGFBI之间差异无统计学意义,P>0.05。结论:TGFBI可以增强胃癌细胞SGC-7901的转移潜能。OBJECTIVE： To study the effect of transforming growth factor beta-induced （TGFBI） on the metastatic potential of SGC-7901. METHODS： The 96-well microculture plates were coated with BSA. Fibronectin （FN） and TGF- BI,on which SGC-7901 was cultured and the adhesion and proliferation was measured by MTT; the undersurface of the membrane of transwell plates was coated with BSA, fibronectin and TGFBI. The ability of migration was measured. RE- SULTS： FN and TGFBI could support SGC-7901 adhesion in a greater degree than that of BSA （P=0. 023）. There was no significant difference between FN and TGFBI （P〉0.05）. The ability of BSA, FN and TGFBI to induce SGC-7901 proliferation was increased in turn （P=0. 031）. FN and TGFBI could induce SGC-7901 migration in a greater degree than that of BSA （P=0. 019）. There was no significant differences between FN and TGFBI （P〉0.05）. CONCLUSION： TG- FBI can improve the metastatic potential of SGC-7901.

关 键 词：转化生长因子Β 胃肿瘤 细胞粘附 细胞增殖 细胞运动 

分 类 号：R735.2[医药卫生—肿瘤]