榄香烯对恶性淋巴瘤细胞多药耐药性影响及机制的研究  

Influence of clemene on MDR of the lymphoma cell and it's mechanism

作　　者：冯爱梅[1] 赵朴[1] 郭海飞[1] 薛阿利[1] 张浩[1] 刘凌云[2] 李强[2] 林峰[2] 

机构地区：[1]温州医学院附属第三医院血液科,浙江温州325200 [2]温州医学院附属第三医院检验科,浙江温州325200

出　　处：《中华肿瘤防治杂志》2012年第15期1149-1152,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：温州市科学技术局资助(2009R0071)

摘　　要：目的:研究榄香烯对恶性淋巴细胞瘤细胞多药耐药性的影响及相关机制。方法:以不同浓度榄香烯作用于恶性淋巴瘤Raji细胞,并设空白对照组。通过RT-PCR以及蛋白质印迹方法检测加药后Raji细胞环氧化酶-2(COX-2)、核因子-κB(NF-κB)的mRNA及COX-2、NF-κB 56蛋白表达情况。结果:24h后,RT-PCR产物经电泳分析,COX-2mRNA、NF-κB mRNA各扩增产物在其相应位置上可见清晰的条带,特异性好。COX-2mRNA、NF-κB mRNA灰度值,各浓度榄香烯处理组与阴性对照组相比差异有统计学意义,P<0.01。各浓度榄香烯处理组与阴性对照组不同程度地表达COX-2、NF-κB p65蛋白,各浓度榄香烯处理组COX-2、NF-κB p65蛋白表达量与阴性对照组相比均下降,呈浓度依赖性,P<0.01。结论:榄香烯可能通过对COX-2和NF-κB的调控影响恶性淋巴细胞瘤细胞多药耐药性。OBJECTIVE： To explore the influence of clemene on the expression of COX-2, NF-κB in Raji cell and it＇ s mechanism. METHODS： The different doeses of clemene were used on Raji cell, comparing to another five contrasting medicine. Experssion of mRNA of COX-2, NF-κB and COX-2, NF-κB p56 proteinum were determined through RT-PCR and Western blot methods. RESULTS： Suppression of all medicines on the expression of mRNA of COX-2, NF-κB after 24 h. There was a difference betweent the different does clemene on the expression of COX-2, NF-κB and 0 μmol/L groups（P〈0.01）. Western blot results showed that a specific protein strip of COX-2 and NF-κB p56 expression was ob- served at the 72 × 10^3 and become more obviously following does（P〈0.01）. CONCLUSION： The ciemene has effect on the expression of COX-2 and NF-κB, and decase the MDR of Raji ceil.

关 键 词：淋巴瘤 抗药性 肿瘤 前列腺素内过氧化物合酶 核因子ΚB 

分 类 号：R733.1[医药卫生—肿瘤] R285[医药卫生—临床医学]