稳定表达荧光素酶裸鼠垂体腺瘤移植瘤模型的建立  

Establishment of pituitary adenoma xenograft models in nude mice with stable luciferase expression

作　　者：张婧惟[1] 魏兰兰[1] 秦颖[1] 童丽萍[1] 纪羽婷[1] 徐坤[2] 初明[2] 

机构地区：[1]哈尔滨医科大学微生物学教研室黑龙江省感染与免疫重点实验室,150086 [2]哈尔滨医科大学附属第一医院神经外科,150001

出　　处：《中国微侵袭神经外科杂志》2012年第9期418-421,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：黑龙江省科技厅留学基金(编号:LC201014);哈尔滨医科大学附属第一医院科研基金(编号:2009L04);黑龙江省科技厅攻关基金项目(编号:ZD201020);哈尔滨市科技局归国留学基金项目(编号:2011RFLXS025)

摘　　要：目的建立稳定表达荧光素酶的裸鼠垂体腺瘤移植瘤模型，并通过活体生物发光成像系统实时观察移植瘤的生长特点。方法经G418药物选择性对转染细胞进行筛选，获得稳定表达荧光素酶的GH3-luc和MMQ-luc细胞系。将两株细胞系分别皮下接种于BALB／c裸鼠，建立BALB／c裸鼠移植瘤模型，利用活体生物发光成像系统观察肿瘤的成瘤和生长情况。结果本组成功构建稳定表达荧光素酶的裸鼠GH3-luc和MMQ-luc移植瘤模型。体表直尺测量显示：移植瘤在接种MMQ-luc和GH3-luc细胞后10d内生长均较缓慢，15—30d肿瘤体积迅速增长．且MMQ-luc移植瘤较GH3-luc移植瘤生长快。活体生物发光成像系统荧光测量显示：接种GH3-luc细胞移植瘤20d时的荧光强度明显高于10d的荧光强度。活体生物发光成像系统荧光测量与取瘤直尺测量的肿瘤体积变化趋于一致，而体表直尺测量的肿瘤体积偏小。结论稳定表达荧光素酶的裸鼠垂体腺瘤移植瘤模型的成功建立，不仅可用于活体生物发光成像系统实时观察肿瘤的成瘤及生长情况，而且为垂体腺瘤的研究及药物开发提供了方便、有效的平台。Objective To establish a pituitary adenoma xenograft models with stable luciferase expression in nude mice, and real-timely observe the growth characteristics of tumor by in vivo bioluminescence imaging system. Methods The transfected GH3-luc and MMQ-luc cells were screened with G418 for selecting the cell line with stable luciferase expression, and the xenograft models were established through subcutaneously inoculating the two cell lines into BALB/c nude mice. The tumorigenicity and tumors growth were observed by in vivo bioluminescence imaging system. Results The GH3-luc and MMQ-luc xenograft models with stable luciferase expression were established successfully in nude mice. Surface ruler measurement showed that the xenograft grew slowly during 10 days after inoculating GH3-luc and MMQ-luc cells, the tumors volume had a rapid growth from 15 to 30 days, and MMQ-luc xenograft grew faster than GH31uc xenograft under the same culture conditions. The in vivo bioluminescence imaging system measurement showed that fluorescence intensity of xenograft tumor inoculated with GH3-1uc was higher on the 20th day than the 10th day. The tumor volume change was similar measured by in vivo bioluminescence imaging system and tumor ruler measurement, and was slightly smaller measured by surface ruler measurement. Conclusions A pituitary adenoma xenograft model with stable luciferase expression is established successfully in nude mice： The model not only can be used to real-timely observe the tumorigenicity and tumor growth by in vivo bioluminescence imaging system, but also provide a convenient and effective platform for research of pituitary adenoma and drug development.

关 键 词：垂体肿瘤 移植瘤 荧光素类 模型 动物 小鼠 裸 

分 类 号：R736.4[医药卫生—肿瘤] R332[医药卫生—临床医学]