Protective effect of low potassium dextran solution on acute kidney injury following acute lung injury induced by oleic acid in piglets  

作　　者：WU Rui-ping LIANG Xiu-bin GUO Hui ZHOU Xiao-shuang ZHAO Li WANG Chen LI Rong-shan 

机构地区：[1]Department of Nephrology, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China [2]Department of Pathology , Second Hospital ofShanxi Medical University, Taiyuan, Shanxi 030001, China [3]Center of Metabolic Disease Research, Nanjing Medical University, Nanjing, Jiangsu 210029, China

出　　处：《Chinese Medical Journal》2012年第17期3093-3097,共5页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （No. 30971380 and No. 31071026）; and by the National Excellent Doctoral Dissertation of China.

摘　　要：Background Low potassium dextran （LPD） solution can attenuate acute lung injury （ALl）. However, LPD solution for treating acute kidney injury secondary to ALl has not been reported. The present study was performed to examine the renoprotective effect of LPD solution in ALl induced by oleic acid （OA） in piglets. Methods Twelve animals that suffered an ALl induced by administration of OA into the right atrium were divided into two groups： the placebo group （n=6） pretreated with normal saline and the LPD group （n=6）, pretreated with LPD solution. LPD solution was injected intravenously at a dose of 12.5 ml/kg via the auricular vein 1 hour before OA injection. Results All animals survived the experiments with mild histopathological injury to the kidney. There were no significant differences in mean arterial pressure （MAP）, creatinin and renal damage scores between the two groups. Compared with the placebo group, the LPD group had better gas exchange parameters at most of the observation points （（347.0±12.6） mmHg vs. （284.3±11.3） mmHg at 6 hours after ALl, P 〈0.01）. After 6 hours of treatment with OA, the plasma concentrations of NGAL and intedeukin （IL）-6 in both groups increased dramatically compared to baseline （（6.0±0.6） and （2.50±0.08） folds in placebo group; and （2.5±0.5） and （1.40±0.05） folds in LPD group）, but the change of both parameters in the LPD group was significantly lower （P 〈0.01） than in the placebo group. And 6 hours after ALl the kidney tissue concentration of IL-6 in the LPD group （（165.7 ± 22.5） pg·m-1·g-1 protein） was significantly lower （P 〈0.01） than that in placebo group （（67.2± 25.3） pg·m-1·g-1 protein）. Conclusion These findings suggest that pretreatment with LPD solution via systemic administration might attenuate acute kidney injury and the cytokine response of IL-6 in the ALl piglet model induced by OA injection.Background Low potassium dextran （LPD） solution can attenuate acute lung injury （ALl）. However, LPD solution for treating acute kidney injury secondary to ALl has not been reported. The present study was performed to examine the renoprotective effect of LPD solution in ALl induced by oleic acid （OA） in piglets. Methods Twelve animals that suffered an ALl induced by administration of OA into the right atrium were divided into two groups： the placebo group （n=6） pretreated with normal saline and the LPD group （n=6）, pretreated with LPD solution. LPD solution was injected intravenously at a dose of 12.5 ml/kg via the auricular vein 1 hour before OA injection. Results All animals survived the experiments with mild histopathological injury to the kidney. There were no significant differences in mean arterial pressure （MAP）, creatinin and renal damage scores between the two groups. Compared with the placebo group, the LPD group had better gas exchange parameters at most of the observation points （（347.0±12.6） mmHg vs. （284.3±11.3） mmHg at 6 hours after ALl, P 〈0.01）. After 6 hours of treatment with OA, the plasma concentrations of NGAL and intedeukin （IL）-6 in both groups increased dramatically compared to baseline （（6.0±0.6） and （2.50±0.08） folds in placebo group; and （2.5±0.5） and （1.40±0.05） folds in LPD group）, but the change of both parameters in the LPD group was significantly lower （P 〈0.01） than in the placebo group. And 6 hours after ALl the kidney tissue concentration of IL-6 in the LPD group （（165.7 ± 22.5） pg·m-1·g-1 protein） was significantly lower （P 〈0.01） than that in placebo group （（67.2± 25.3） pg·m-1·g-1 protein）. Conclusion These findings suggest that pretreatment with LPD solution via systemic administration might attenuate acute kidney injury and the cytokine response of IL-6 in the ALl piglet model induced by OA injection.

关 键 词：low potassium dextran solution acute kidney injury acute lung injury interleukin-6 PIGLET 

分 类 号：R[医药卫生]