慢性轻度不可预见性应激抑郁模型大鼠血清同型半胱氨酸和抗环瓜氨酸肽抗体浓度升高  

Concentration increase in Hcy and anti-CCP antibody in the serum of depression rat model induced by chronic unpredictable mild stress

作　　者：唐亚梅[1] 刘勇[2] 秦立新[1] 张仁生[1] 陈若虹[1] 

机构地区：[1]中南大学湘雅二医院检验科,长沙410011 [2]中南大学湘雅二医院精神卫生研究所,长沙410011

出　　处：《中南大学学报（医学版）》2012年第8期790-795,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81171268)~~

摘　　要：目的:观察慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)诱导的抑郁模型大鼠血清同型半胱氨酸(homocysteine,Hcy)和抗环瓜氨酸肽(anti-citrullinated peptide,anti-CCP)抗体浓度的变化,探讨抑郁症的免疫机制及其与自身免疫的关系。方法:60只成年雄性SD大鼠随机分为2大组,每组30只,每大组再分为正常对照组、模型组和氟西汀(uoxetine,Flx)治疗组3个亚组。以CUMS结合孤养建立抑郁大鼠模型,造模完成后,第一大组大鼠用于旷场试验、糖水消耗试验和强迫游泳试验;第二大组大鼠心脏穿刺采血后,以循环酶法测定血清Hcy水平,ELISA法测定血清anti-CCP抗体浓度。结果:与对照组和氟西汀组比较,模型组大鼠自发活动显著减少;糖水消耗量、糖水偏爱百分比显著降低;强迫游泳不动时间明显延长;血清Hcy和anti-CCP抗体水平明显升高。结论:CUMS抑郁模型大鼠体内存在免疫炎症和自身免疫反应,氟西汀治疗可改善上述免疫反应。Objective： To observe the change of serum homocysteine（Hcy） and anti-citrullinated peptide（CCP） antibody concentration in depression rat model induced by chronic unpredictable mild stress（CUMS）,and to explore the immunologic mechanism of depression and the relation between depression and its autoimmunity. Methods： Sixty adult male SD rats were randomly divided into 2 groups,30 rats in each group,which were divided into 3 subgroups： a normal control group,a model group and a fluoxetine-treated group.The depression rat model was established under CUMS and seperated feeding,after which,open field,sugar consumption and forced swimming test were applied in the first group.After the blood was taken in the second group of rats through heart puncture,the level of serum Hcy was detected by enzymatic cycling assay and serum anti-CCP antibody by ELISA. Results： Compared with the control group and the fluoxetine treatment group,spontaneous activity and sucrose consumption and preference percentage of the rats in the model group significantly reduced,while the immobility time in forced swimming test and the level of Hcy and anti-CCP antibody in the rat serum significantly increased. Conclusion： Immunity inflammation and autoimmune reaction exist in CUMS depression model rats,and fluoxetine treatment can improve these immune response.

关 键 词：抑郁症 CUMS 免疫 同型半胱氨酸 anti-CCP抗体 

分 类 号：R749.4[医药卫生—神经病学与精神病学]