烟酰胺磷酸核糖转移酶、血管内皮生长因子A在胃癌组织中的表达及临床意义  

The expression of nicotinamide phosphoribosyl transferase and vascular endothelial growth factor-A in gastric carcinoma and their clinical significance

作　　者：龙厚隆[1] 车向明[1] 毕铁强[1] 李海军[1] 刘俊松[1] 李铎伟[1] 

机构地区：[1]西安交通大学医学院第一附属医院普通外科,710061

出　　处：《中华外科杂志》2012年第9期839-842,共4页Chinese Journal of Surgery

摘　　要：目的探讨胃癌中烟酰胺磷酸核糖转移酶（Nampt）、血管内皮生长因子A（VEGF—A）的表达与临床病理特性的关系及对患者预后的影响。方法采用免疫组化方法检测2000年1月至2004年12月手术治疗的68例患者胃癌组织和其中59例癌旁正常胃黏膜组织中Nampt、VEGF—A的表达情况，并对68例胃癌患者的生存情况进行随访。结果Nampt在正常胃黏膜（6±3）及胃癌组织（13±5）中均表达，但胃癌组织表达显著升高（t=7．46，P〈0．01）；胃癌中Nampt的表达程度与浸润深度（F=4．693，P=0．034）、淋巴结转移（F=4．027，P=0．049）及TNM分期（F=9．979，P=0．002）相关，与患者性别、年龄、肿瘤部位、大小及组织分化程度无关（P〉0．05）。胃癌中Nampt的表达与患者术后生存有关，Nampt表达升高，患者预后变差，Nampt、VEGF—A共表达升高，则患者预后更差。Nampt与VEGF—A在胃癌中的表达呈正相关（r=0．293，P=0．015）。结论Nampt与VEGF—A的表达呈正相关；两者可能在促进肿瘤发展、转移的功能上具有一定的协同性。Objectives To study the expression of nicotinamide phosphoribosyl transferase （Nampt） and vascular endothelial growth factor-A （VEGF-A） in gastric carcinoma and investigate their correlations to clinicopathologic features and prognostic significance. Methods The proteins of Nampt and VEGF-A in 68 specimens of gastric carcinoma and 59 specimens normal gastric tissue were detected by immunohistochemistry during January 2000 to December 2004, and the 68 patients were followed up. Results Nampt protein was detected in the cytoplasm of both tissues, and Nampt in gastric carcinoma （ 13 ＋ 5） were significantly higher than that in normal gastric tissue （6 -＋- 3 ） （ t = 7.46, P 〈 0. 01 ）. The expression of Nampt was correlated to invasive depth （ F -- 4. 693, P = 0. 034 ） , lymph node metastasis （F =4. 027, P = 0. 049 ） , clinical TNM stage （ F = 9. 979, P = 0. 002 ） , but not to gender, age, tumor location,tumor size, differentiation （ P 〉 0. 05 ） . The expression of Nampt is correlated with survival of patients that underwent surgical resection for gastric cancer. The survival rate of patients in negative of Nampt was very higher than that of the positive patients, and its co-expression with VEGF-A showed a trend towards poorer survival. The positive correlation was found between the expression of Nampt and VEGF-A in gastric carcinoma （r = 0. 293, P = 0. 015）. Conclusions The expression of Nampt is positively correlated to that of VEGF-A in gastric carcinoma. The correlation between the expression of Nampt and VEGF-A in gastric carcinoma plays an important role cooperatively in carcinogenesis, development and metastasis of gastric carcinoma.

关 键 词：胃肿瘤 烟酰胺磷酸核糖基转移酶 血管内皮生长因子A 免疫组织化学 预后 

分 类 号：R735.2[医药卫生—肿瘤]