Sonic hedgehog elevates N-myc gene expression in neural stem cells  

作　　者：Dongsheng Liu Shouyu Wang Yan Cui Lun Shen Yanping Du Guilin Li Bo Zhang Renzhi Wang 

机构地区：[1]Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China [2]Chinese Academy of Medical Sciences, Department of Neurosurgery, Peking Union Medical College Hospital, Beijing 100730, China

出　　处：《Neural Regeneration Research》2012年第22期1703-1708,共6页中国神经再生研究（英文版）

基　　金：funded by the National Natural Science Foundation of China,No.81171401;Science and Technology Development Program of Dalian City,No.2008J99JH268;the Scientific Research Program of Higher Learning School of Department of Education of Liaoning Province,No.L20100108

摘　　要：Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gill and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gill and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

关 键 词：stem cells neural stem cells sonic hedgehog signal pathway N-myc gene PROLIFERATION targetgene neural regeneration 

分 类 号：Q421[生物学—神经生物学] TS67[生物学—生理学]