SDF-1/CXCR4的表达与喉癌组织VEGF-C表达、淋巴管生成及预后的相关性  

Correlation between SDF-1,CXCR4 and VEGF-C,Lymphangiogenesis,Prognosis in Laryngeal Carcinoma

作　　者：王莹[1] 李文媛[1] 梁金花[2] 丁利[1] 赵斯达[1] 

机构地区：[1]牡丹江医学院解剖教研室,157011 [2]牡丹江医学院红旗医院检验科

出　　处：《医学研究杂志》2012年第8期50-54,共5页Journal of Medical Research

基　　金：黑龙江省卫生厅科研基金资助项目(2010243);黑龙江省普通高等学校青年学术骨干支持计划项目(1152G050)

摘　　要：目的探讨趋化因子SDF-1及其受体CXCR4在喉癌组织中的表达与喉癌中VEGF-C表达、淋巴管生成及预后的相关性。方法应用免疫组化法检测SDF-1、CXCR4和VEGF-C在45例喉癌和20例声带息肉组织中的表达,应用5'-核苷酸酶染色法(5'-Nase)计数淋巴管密度,并结合临床病理特征进行相关分析,应用Kaplan-Meier法评估SDF-1和CXCR4表达对喉癌预后的影响。结果喉癌组织中SDF-1、CXCR4和VEGF-C表达较声带息肉组显著增加(P<0.05),SDF-1、CXCR4表达与TNM分期、淋巴结转移、淋巴管浸润相关(P<0.05),VEGF-C表达与淋巴结转移、淋巴管浸润相关(P<0.05)。SDF-1、CXCR4和VEGF-C表达呈正相关(P<0.05),三者均与瘤内和瘤周淋巴管密度相关(P<0.05)。SDF-1阳性表达生存率显著低于阴性表达(P<0.05)。结论 SDF-1/CXCR4生物轴可能通过上调VEGF-C表达促进喉癌淋巴管生成和淋巴结转移,SDF-1高表达是喉癌预后不良的重要指标。Objective To investigate the relationship between the expression of SDF - 1 ,CXCR4 and VEGF - C, lymphangiogene- sis, prognosis significance in patients with laryngeal cancer. Methods The expressions of SDF - 1, CXCR4 and VEGF - C in 45 speci- mens of the laryngeal cancer and 20 specimens of polyp of vocal cord tissues were observed by immunohistochemistry. Lymphatic mi- crovessed density（LVD） was evaluated by 5＇ - Nase staining, and the correlation analysis was made between SDF - 1 , CXCR4 and VEGF - C, clinical characteristics, and judged SDF - 1 and CXCR4 roles in laryngeal cancer prognosis by using Kaplan - Meier method. Re- suits The expression of SDF - 1, CXCR4, VEGF - C protein were higher in laryngeal cancer tissue than in the polyp of vocal cord tis- sue. The expression of SDF - 1 and CXCR4 were significantly correlated with TNM stage, lymph node metastasis, lymphovaseular inva- sion. VEGF - C was correlated with lymph node metastasis, lymphovascular invasion. The expression of the SDF - 1, CXCR4 and VEGF - C protein in laryngeal cancer has directly correlation. SDF - 1, CXCR4, VEGF - C were significantly correlated with peritumoral and in- tratumoral LVD. The expression of SDF - 1 had effect on survival ratio. Conclusion SDF - 1/CXCR4 axis might stimulate VEGF - C up- regulation and promote lymphangiogenesis, lymphatic metastasis in laryngeal cell carcinoma. The expression of SDF - 1 may be helpful for judging prognosis in laryngeal cancer.

关 键 词：趋化因子基质细胞衍生因子1 CXC趋化因子受体 血管内皮生长因子-C 喉癌 

分 类 号：R730-36[医药卫生—肿瘤]