直接和间接共培养条件下人脐带间充质干细胞对恶性胶质瘤细胞系SHG44生长的抑制作用  

Human umbilical cord mesenchymal stromal cells inhibit the growth of SHG44 cells under direct and indirect co-cultivation

作　　者：梁阿铭[1,2] 梁硕 樊锐太[4] 田毅[1,2] 迟连凯[3] 史辛艺[3] 刘金瑜[1,2] 关方霞[3] 杨波[1,2] 

机构地区：[1]郑州大学第一附属医院神经外科,河南省郑州市450052 [2]河南省高等学校临床医学重点学科开放实验室,河南省郑州市450052 [3]郑州大学生物工程系,河南省郑州市450001 [4]郑州大学第一附属医院放疗科,河南省郑州市450052

出　　处：《中国组织工程研究》2012年第32期5891-5896,5893+5892+5894-5896,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南省科技攻关项目(112102310173);河南省杰出青年基金项目编号(114100510005);郑州大学211三期建设项目"干细胞基础与临床研究;郑州大学第一附属医院创新科研团队建设基金项目~~

摘　　要：背景:SHG44是一种恶性胶质瘤细胞系,其生物学性状稳定、易于培养,广泛应用于动物成瘤模型制作,目前对其研究主要集中在运用药物、基因转染及电离辐射等途径抑制其生长,未见间充质干细胞对其作用的相关报道。目的:通过直接和间接共培养的方法,探讨人脐带间充质干细胞对恶性胶质瘤细胞系SHG44的作用,观察SHG44胶质瘤细胞数量及细胞周期的变化。方法:将人脐带间充质干细胞与SHG44细胞,分别在24孔板进行直接共培养,在Transwell板进行间接共培养,分别设对照组。培养3d后在荧光显微镜下观察,并收集Transwell板中的SHG44胶质瘤细胞,应用流式细胞术分别检测SHG44细胞周期。结果与结论:直接接触共培养组SHG44胶质瘤细胞数量少于对照组(P<0.05)。Transwell间接接触共培养组SHG44胶质瘤细胞数量与对照组相比,差异无显著性意义(P>0.05),其细胞周期位于G0/G1期的SHG44胶质瘤细胞比率高于对照组(P<0.05)。证实,体外培养的人脐带间充质干细胞可以通过直接及间接接触抑制恶性胶质瘤细胞系SHG44细胞的生长。BACKGROUND：SHG44, a kind of malignant glioma cell line, is easy to cultivate with stable biomedical properties and widely used in the animal models of tumors. The current studies focus on inhibiting SHG44 growth by drugs, gene transfection and ionizing radiation. There have been no reports describing the effects of mesenchymal stem cells on biological characteristics of SHG44. OBJECTIVE：To investigate the cell cycle and the number of SHG44 cells which were directly and indirectly co-cultured with human umbilical cord mesenchymal stem cells. METHODS：Human umbilical cord mesenchymal stem cells and SHG44 cells were directly co-cultured in the 24-well plate and indirectly cocultured in the Transwell plate. A control group was designed for the direct and indirect co-culture separately. After co-culture for 3 d, cells were observed under fluorescence microscope, and SHG44 cells were collected for detection of SHG44 cell cycle by flow cytometry. RESULTS AND CONCLUSION：Compared with corresponding controls, the number of SHG44 cells fluorescently labeled decreased after directly co-cultured with MSCs （P 0.05）; the number of SHG44 cells fluorescently labeled had no change after indirectly co-cultured with MSCs （P 0.05）. The proportion of SHG44 cells at G0/G1 stage was significantly higher than that in the control group （P 0.05）. The results confirmed that human umbilical cord mesenchymal stem cells cultured in vitro can inhibit the proliferation of SHG44 cells by direct and indirect ways in vitro.

关 键 词：直接共培养 间接共培养 脐带间充质干细胞 SHG44细胞 抑制作用 恶性胶质瘤细胞 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]