人脐带间充质干细胞表达胚胎干细胞相关转录因子  

Expression of embryonic stem cell-related transcription factors in human umbilical cord mesenchymal stem cells

作　　者：韩之波 池颖 杨少光 王有为[1,3] 杨舟鑫 及月茹 杨萍 韩忠朝[1,2,3] 

机构地区：[1]中国医学科学院血液学研究所血液病医院实验血液学国家重点实验室,天津市300020 [2]中国医学科学院血液学研究所泰达生命科学研究中心,天津市300457 [3]细胞产品国家工程研究中心,天津市300457

出　　处：《中国组织工程研究》2012年第32期5897-5902,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家高技术研究发展计划"863计划"项目(2011AA020118)~~

摘　　要：背景:Nanog、Oct4和Sox2通过调节胚胎干细胞的基因转录,对其多潜能性和自我更新的能力具有关键性的调控作用,脐带间充质干细胞中这些胚胎干细胞相关转录因子的表达情况如何还不太清楚。目的:研究脐带间充质干细胞中Nanog、Oct4和Sox2等这些胚胎干细胞相关转录因子的表达情况。方法:胶原酶和胰酶消化法培养脐带间充质干细胞;mTeSRTM1体系进行无滋养层培养人胚胎干细胞,定量PCR比较上述两种细胞中Nanog、Oct4和Sox2 mRNA表达量的差异;免疫荧光检测上述两种细胞中Nanog、Oct4和Sox2的表达情况。结果与结论:间充质干细胞表达胚胎干细胞标记Nanog、Oct4和Sox2,但Oct4主要表达在胞浆,且以Oct4B为主。脐带间充质干细胞Nanog、Oct4A和Sox2的表达量明显低于胚胎干细胞,其mRNA表达量分别为胚胎干细胞的20%,0.3%,10%左右。通过了解两种细胞Nanog、Oct4和Sox2的表达差异,可为优化脐带间充质干细胞重编程提供依据,也为进一步研究胚胎干细胞相关转录因子在成体干细胞表达起何种作用提供参考。BACKGROUND： By regulating gene transcription in embryonic stem cells, Nanog, Oct4 and Sox2 play a key role in the regulation of pluripotency and self-renewal. Expression of embryonic stern cell-associated transcription factors in umbilical cord mesenchymal stem cells has not been fully clarified. OBJECTIVE： To investigate the expression of embryonic stem cell-associated transcription factor Nanog, Oct4 and Sox2 in umbilical cord mesenchymal stem cells. METHODS： Umbilical cord mesenchymal stem cells were cultured with collagenase and trypsinase digestion. Human embryonic stem cells were cultured in the mTeSRTM1 system in the absence of trophoderm. The difference in Nanog, Oct4 and Sox2 mRNA expression was compared between umbilical cord mesenchymal stem cells and embryonic stem cells by quantitative PCR. Nanog, Oct4 and Sox2 expression in umbilical cord mesenchymal stem cells and embryonic stem cells was determined by immunofluorescent staining. RESULTS AND CONCLUSION： Nanog, Oct4 and Sox2 were expressed in human umbilical cord mesenchymal stem cells. However, Oct4, in particular Oct4B, was mainly located in the cytoplasm of human umbilical cord mesenchymal stem cells. Nanog, Oct4 and Sox2 expression was significantly lower in the umbilical cord mesenchymal stem cells than in the embryonic stem cells, and the mRNA expression of Nanog, Oct4 and Sex2 in the umbilical cord mesenchymal stem cells was 20%, 0.3% and 10% of that in the embryonic stem cells. This study revealed the different expression profiles of Nanog, Oct4 and Sox2 in umbilical cord mesenchymal stem cells and embryonic stem cells. It is beneficial to optimizing the methods of reprogramming umbilical cord mesenchymal stem cells and analyzing the function of embryonic stem cell-associated transcription factors in adult stem cells.

关 键 词：脐带间充质干细胞 胚胎干细胞 转录因子 NANOG OCT4 SOX2 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]