胶质细胞源性神经营养因子基因修饰骨髓基质干细胞移植脑出血大鼠神经营养因子的表达  

Expression of neurotrophic factors in rats with intracerebral hemorrhage following transplantation of bone marrow stromal cells modified by glial cell line-derived neurotrphic factor gene

作　　者：邓莉[1] 常能彬[2] 熊怀林[2] 高小青[1] 杨朝鲜[1] 

机构地区：[1]泸州医学院神经生物学研究室,四川省泸州市646000 [2]泸州医学院解剖教研室,四川省泸州市646000

出　　处：《中国组织工程研究》2012年第32期6019-6024,共6页Chinese Journal of Tissue Engineering Research

基　　金：四川省卫生厅科研基金项目(100224);泸州医学院青年基金~~

摘　　要：背景:研究证实,细胞移植和神经营养因子相结合治疗脑损伤能促进大鼠神经功能的恢复。目的:观察移植胶质细胞源性神经营养因子基因修饰的骨髓基质干细胞对大鼠脑出血后神经营养因子表达的影响。方法:通过脑立体定位仪向SD大鼠脑尾壳核注射胶原酶和肝素建立脑出血动物模型,将48只模型鼠随机分为3组,骨髓基质干细胞组、胶质细胞源性神经营养因子/骨髓基质干细胞组和对照组于建模后第3天在脑出血部位分别移植骨髓基质干细胞、胶质细胞源性神经营养因子/骨髓基质干细胞以及生理盐水。结果与结论:与对照组和骨髓基质干细胞组相比,胶质细胞源性神经营养因子/骨髓基质干细胞组大鼠神经功能恢复更好;与对照组相比,移植后1,2周其他2组各神经营养因子表达均显著增加(P<0.05)。提示胶质细胞源性神经营养因子基因修饰的骨髓基质干细胞移植治疗脑出血大鼠比单纯骨髓基质干细胞有更好的神经保护作用。BACKGROUND：Previous studies have demonstrated that cell transplantation combined with neurotrophic factor can promote neural function recovery after brain injury. OBJECTIVE：To explore the effects of transplantation of bone marrow stromal cells （BMSCs） modified by glial cell line-derived neurotrphic factor （GDNF） gene on expression of neurotrophic factors in rats with intracerebral hemorrhage. METHODS：Forty-eight rats were used to establish the models of intracerebral hemorrhage by injecting with collagenase and heparin into caudate nucleus through a stereotaxic apparatus. Then rats were randomly divided into BMSCs group, BMSCs/GDNF group and saline group and were stereotaxically injected with BMSCs, GDNF/BMSCs and saline respectively at the 3rd day after intracerebral hemorrhage induction. RESULTS AND CONCLUSION：Compared with saline group and BMSCs group, neurological function recovery was better in the GDNF/BMSCs group （P 0.05）. At 1, 2 weeks after cell transplantation, the expression of neurotrophic factors in the BMSCs group and GDNF/BMSCs group was significantly increased than in the saline group （P 0.05）. Transplantation of BMSCs modified by GDNF gene provides better neuroprotective effects than transplantation of simple BMSCs in treatment of intracerebral hemorrhage.

关 键 词：胶质细胞源性神经营养因子 骨髓基质干细胞 脑出血 神经营养因子 基因治疗 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]