抗阻训练衰老小鼠骨骼肌p53与bax基因的表达  

Effect of resistance training on p53 and bax gene expression in the skeletal muscle of aging mice

作　　者：王立丰[1] 李海鹏[2] 卢健[1] 

机构地区：[1]华东师范大学体育与健康学院,上海市200241 [2]上海体育学院运动人体科学学院,上海市200081

出　　处：《中国组织工程研究》2012年第33期6127-6131,共5页Chinese Journal of Tissue Engineering Research

基　　金：教育部博士点基金项目(200802690017)~~

摘　　要：背景:抗阻训练能够缓解肌肉衰减征的发生并能够弱化骨骼肌细胞凋亡。目的:观察年龄和抗阻训练对SAMP8小鼠骨骼肌p53,bax基因表达的影响。方法:分别取3月龄(青年组)和6月龄(老年组)的SAMP8小鼠进行8周爬梯运动,每周3次,以不参加运动的同龄小鼠作为对照。结果与结论:Real-time PCR结果显示,与青年组比较,老年组小鼠骨骼肌纤维p53,baxm RNA的表达量显著上升(P<0.05),而与相同月龄对照组比较,抗阻训练的小鼠骨骼肌纤维中p53,bax mRNA的表达量明显降低(P<0.05)。提示抗阻训练能抑制衰老小鼠骨骼肌p53,bax基因的表达。BACKGROUND： Resistance training can alleviate muscle attenuation and weaken the apoptosis of skeletal muscle cells OBJECTIVE： To observe the effects of aging and resistance training on the expression of p53 and bax genes in the skeletal muscle of SAMP-8 mice. METHODS： SAMP-8 mice aged 3 months and 6 months were selected to undergo 8-week ladder movement （three times per week）. Another mice at the same age without movement served as controls. RESULTS AND CONCLUSION： Real-time PCR showed that p53 and bax mRNA expression increased significantly in the skeletal muscle fibers of 3-month-old mice than 6-month-old mice （P 〈 0.05）. However, compared with those at the same age, p53 and bax mRNA expression in the skeletal muscle fibers decreased significantly in mice undergoing ladder movements （P 〈 0.05）. These findings indicate that resistance movements can suppress p53 and bax gene expression in the skeletal muscle of aging mice.

关 键 词：肌肉衰减征 细胞凋亡 骨骼肌纤维 P53 BAX 抗阻训练 

分 类 号：R318[医药卫生—生物医学工程]