体外贴壁和微囊化生长睾丸支持细胞形态与其功能的相关性  

Correlation between the morphologies and physiological function of adherent and microencapsulated Sertoli cells

作　　者：张国境[1] 赵佳[2] 金洁[1] 路欣[2] 史小林[2] 

机构地区：[1]首都医科大学燕京医学院组织胚胎学教研室,北京市101300 [2]首都医科大学基础医学院组织胚胎学教研室,北京市100069

出　　处：《中国组织工程研究》2012年第33期6189-6193,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:细胞体外培养一直是研究活细胞的主要方法,但体外培养细胞的形态和生长环境与体内环境有很大差异,因此,细胞的生物活性是否能准确反映体内该细胞的状态尚不清楚。目的:观察体外培养细胞的形态与其生理功能间的相关性。方法:体外培养不同生长状态的小鼠睾丸支持细胞,一种为贴壁生长状态,细胞呈扁平状;另一种为微囊化生长状态,细胞呈立体球状。分别取2种不同形态睾丸支持细胞的培养液,进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳,获取相应的蛋白质条带,通过蛋白质相对分子质量的计算来确认睾丸支持细胞分泌蛋白质的种类。结果与结论:贴壁生长的扁平状的睾丸支持细胞培养液中可辨别出14个条带,蛋白质相对分子质量分布在(17～158)×103之间;微囊化生长的立体球状睾丸支持细胞培养液中可辨别出10个条带,蛋白质相对分子质量分布在(17～58)×103之间。提示不同形态的睾丸支持细胞在蛋白质分泌数量和种类上都存在着差异,细胞的形态与其生理功能之间存在着明显的相关性。BACKGROUND： In vitro culture has been a main method for studying the living cells. However, there is a big difference in the morphology of the cells and growth environment between in vitro and in vivo. So it is unclear whether the bioactivity of cells in vitro can reflect precisely the cell growth in vivo. OBJECTIVE： To investigate the correlation between the morphologies of the cells cultured in vitro and their physiological function. METHODS： Different morphologies of mouce Sertoli cells were cultured in vitro. One of morphologies was the growth of cell adherence, and the cells were fiat; another cells grew in microcapsule, which showed sphere. Culture solution from the two kinds of morphologies of SCs was taken out, and sodium dodecyl sulfate polyacrylamide gel electrophoresis was finished for obtaining the corresponding electrophoretic band. And then, the molecular weight of protein was calculated to ascertain which kind of protein secreted by Sertoli cells with different morphologies. RESULTS AND CONCLUSION： There were 14 electrophoretic bands from the growth of cell adherence with flat Sertoli cells, and the molecular weight of protein distributed from 17~ 103 to 158x 103. There were 10 electrophoretic bands from the cell growth in microcapsule with spherical Sertoli cells, and the molecular weight of protein distributed from 17x 103 to 58x 103. These findings suggest that there are differences between the quantity and the kind of protein which is secreted by Sertoli cells with different morphologies. There are obvious correlations between the morphologies of the cells cultured in vitro and their physiological function.

关 键 词：睾丸支持细胞 条件培养液 体外培养 贴壁 微囊 形态 

分 类 号：R318[医药卫生—生物医学工程]