中药浮海石致突变的实验研究  

Experimental Study of Traditional Chinese Medicine Pumex Mutagenic

作　　者：孙洪然 

机构地区：[1]山东省诸城中医医院眼科,山东诸城262200

出　　处：《中国医药指南》2012年第23期408-409,共2页Guide of China Medicine

摘　　要：目的 研究中药浮海石致突变性.方法 本试验采用鼠伤寒沙门菌回复突变试验（Ames 试验）,哺乳动物培养细胞（CHL）染色体畸变试验和小鼠骨髓细胞微核试验观察了浮海石的致突变作用.结果 浮海石生理盐水浸提液0.5~5000μg/ 皿剂量下Ames 试验结果阴性;250~1000μg/mL 剂量下对CHL 细胞无损伤作用,10~40g/kg 剂量下对小鼠骨髓细胞染色体无致畸变作用.结论 浮海石无致突变作用,用于临床是安全的.Objective To study the Chinese traditional medicine pumex mutagenicity. Method this test using Salmonella typhimurium reverse mutation test （ Ames test）, cultured mammalian cells （ CHL ） and chromosome aberration test of mouse bone marrow cell micronucleus test on pumex mutation. Results Pumex saline extraet0.5-5000p, g/dish dose Ames test result was negative; 250-10001zg/mL dose of CHL cells without damage,10-40g / kg dose on mouse bone marrow cell chromosome aberration induced by. Conclusion Pumex has no mutagenic effect, is safe for clinical.

关 键 词：中药海浮石 致突变性 实验研究 

分 类 号：R77[医药卫生—眼科]