Enhanced oxidation of benzo[a]pyrene by crude enzyme extracts produced during interspecific fungal interaction of Trametes versicolor and Phanerochaete chrysosporium  

作　　者：Linbo Qian Baoliang Chen 

机构地区：[1]Department of Environmental Science, Zhejiang University, Hangzhou 310058, China. [2]Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China

出　　处：《Journal of Environmental Sciences》2012年第9期1639-1646,共8页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.41071210,20890111);the National High-Tech Research and Development(863) Program of China(No.2012AA06A203);the Provincial Natural Science Foundation of Zhejiang(No.R5100105);the Doctoral Fund of the Ministry of Education of China(No.J20091588)

摘　　要：The effects of interspecific fungal interactions between Trametes versicolor and Phanerochaete chrysosporium on laccase activity and enzymatic oxidation of polycyclic aromatic hydrocarbons （PAHs） were investigated. A deadlock between the two mycelia rather than replacement of one fungus by another was observed on an agar medium. The laccase activity in crude enzyme extracts from interaction zones reached a maximum after a 5-day incubation, which was significantly higher than that from regions of T. versicolor or P. chrysosporium alone. The enhanced induction of laccase activity lasted longer in half nutrition than in normal nutrition. A higher potential to oxidize benzo[a]pyrene by a crude enzyme preparation extracted from the interaction zones was demonstrated. After a 48 hr incubation period, the oxidation of benzo[a]pyrene by crude enzyme extracts from interaction zones reached 26.2%, while only 9.5% of benzo[a]pyrene was oxidized by crude extracts from T. versicolor. The oxidation was promoted by the co-oxidant 2,2＇-azinobis-3- ethylbenzthiazoline-6-sulphonate diammonium salt （ABTS）. These findings indicate that the application of co-culturing of white-rot fungi in bioremediation is a potential ameliorating technique for the restoration of PAH-contaminated soil.The effects of interspecific fungal interactions between Trametes versicolor and Phanerochaete chrysosporium on laccase activity and enzymatic oxidation of polycyclic aromatic hydrocarbons （PAHs） were investigated. A deadlock between the two mycelia rather than replacement of one fungus by another was observed on an agar medium. The laccase activity in crude enzyme extracts from interaction zones reached a maximum after a 5-day incubation, which was significantly higher than that from regions of T. versicolor or P. chrysosporium alone. The enhanced induction of laccase activity lasted longer in half nutrition than in normal nutrition. A higher potential to oxidize benzo[a]pyrene by a crude enzyme preparation extracted from the interaction zones was demonstrated. After a 48 hr incubation period, the oxidation of benzo[a]pyrene by crude enzyme extracts from interaction zones reached 26.2%, while only 9.5% of benzo[a]pyrene was oxidized by crude extracts from T. versicolor. The oxidation was promoted by the co-oxidant 2,2＇-azinobis-3- ethylbenzthiazoline-6-sulphonate diammonium salt （ABTS）. These findings indicate that the application of co-culturing of white-rot fungi in bioremediation is a potential ameliorating technique for the restoration of PAH-contaminated soil.

关 键 词：white-rot fungus interspecific interaction laccase activity BENZO[A]PYRENE OXIDATION 

分 类 号：X53[环境科学与工程—环境工程] TS201.2[轻工技术与工程—食品科学]