REM睡眠对幼鼠海马突触可塑性影响的分子机制研究  

Study of molecular mechanism on effect of rapid eye movement sleep on synaptic plasiticity in rats hippocampus

作　　者：戴斐[1] 丁瑜[1] 瞿飞[1] 田英[1] 李生慧[1] 

机构地区：[1]上海交通大学医学院,上海200025

出　　处：《中国儿童保健杂志》2012年第9期806-809,共4页Chinese Journal of Child Health Care

基　　金：国家自然科学基金面上项目(81072314;30700670);上海市教委科研创新项目(13YZ034);2009年度上海交通大学医学院新百人计划;2012年度上海市公共卫生优秀学科带头人培养计划(GWDTR201222)

摘　　要：【目的】观察幼鼠快速眼动(rapid eye movement,REM)睡眠剥夺及恢复过程中海马GAP-43表达水平,探讨睡眠影响学习记忆功能的突触前机制。【方法】将30只雄性Sprague-Dawle幼鼠随机分为5组:空白对照组、环境对照组、48hREM睡眠剥夺组、睡眠恢复24h组、睡眠恢复48h组;每组6只。采用多平台改良法建立幼鼠REM睡眠剥夺及睡眠恢复模型。应用RT-PCR技术和Western-blot技术分别检测GAP-43基因和蛋白表达水平;并对幼鼠海马切片进行免疫荧光组化分析。【结果】48h睡眠剥夺组Gap-43基因表达显著高于其他各组(P<0.05);该组GAP-43蛋白及其水解片断GAP-43-3在海马组织的表达量上升,但磷酸化程度下降;而睡眠恢复组的GAP-43蛋白及其水解片断GAP-43-3在海马组织的表达量下降,磷酸化程度有所上升;同时,48hREM睡眠剥夺组和恢复睡眠24h组的幼鼠海马组织GAP-43分布范围扩大。【结论】REM睡眠于转录、翻译和翻译后修饰三个层次作用于GAP-43,影响其功能状态,从而实现对学习记忆功能的突触前调控作用。[Objective] To investigate the change of GAP-43 expressed in rats hippocampus after rapid eye move- ment （REM） sleep deprivation and the sleep recovery, and further to explore the possible pre-synaptic mechanisms of learn- ing and memory. [Methods] Male Sprague-Dawley rats （n=30） were divided randomly into 5 groups： blank control group, environment control group, 48 h REM sleep deprivation group, sleep recovery group （24 h） , and sleep recovery group （48 h）. The REM sleep deprivation model was established by the modified multiple platform methods. The gene and protein expression of GAP-43 were detected by RT-PCR and Western-blot. Hippocampus slices in rats were observed by immunohistochemistry method. [Results] The expression of Gap-43 gene in 48 h sleep deprivation group was signifi- cantly higher than that of the other groups （P~ 0.05）. The expression of GAP-43 protein and its hydrolysis fragment, GAP-43-3 were increased, and the expression of phoslghi3rylation were decreased. But in sleep recovery groups, the expres- sion of GAP-43 protein and GAP-43-3 were decreased, and the expression of phosphorylation were increased. At the same time, the distribution of GAP-43 in rats hippocampus were expanded in 48 h REM sleep deprivation group and 24 h recovery sleep group. [Conclusions] The level of GAP-43 expression in rats hippocampus are affected by REM sleep through three ways, transcription, translation and post-translational modification. And finally result in the function of presynaptic regulation in learning and memory.

关 键 词：REM睡眠剥夺 GAP-43 突触可塑性 大鼠 

分 类 号：R749.71[医药卫生—神经病学与精神病学]