Survivin和环氧合酶-2在壶腹癌中的表达及临床意义  

Expression and clinical significance of Survivin and COX-2 in human ampullary carcinoma

作　　者：薛栋[1] 左凯[2] 李新军[3] 王建强[1] 张成德[1] 成丕光[1] 张同军[1] 巩本刚[1] 

机构地区：[1]山东省滨州市人民医院肝胆外科,256610 [2]山东省滨州市人民医院感染二科,256610 [3]山东省滨州市人民医院病理科,256610

出　　处：《国际肿瘤学杂志》2012年第9期713-717,共5页Journal of International Oncology

摘　　要：目的探讨Survivin和环氧合酶-2〈COX-2）蛋白在壶腹癌组织中的表达及其临床意义。方法用免疫组化EnVision法对40例壶腹癌组织进行Survivin和COX-2蛋白检测，并对8例正常壶腹组织进行对照研究。结果壶腹癌组织中33例Survivin蛋白呈阳性表达，阳性率为82．5％，对照组中无阳性表达，两组阳性率比较，差异有统计学意义（P〈0．01）；Survivin表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性（均P〈0．05）。在壶腹癌组中27例COX-2蛋白阳性表达，阳性率为67．5％，而对照组8例无阳性表达，两组阳性率比较差异有统计学意义（P〈0．01）；COX-2表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性（均P〈0．05）。经Spearman等级相关性分析表明，Survivin与COX-2蛋白表达呈正相关（r=0．383，P=0．015）。结论Survivin基因和COX-2基因的异常表达可能对壶腹癌的发生和发展起重要作用，可能为壶腹癌的早期诊断和基因治疗提供新的靶点。] Objective To study the expression of Survivin and COX-2 in ampullary carcinoma and their clinical significance. Methods The expression of Survivin and COX-2 proteins were tested by EnVision immunohistochemistry in 40 cases of ampullary carcinomas, and 8 cases of normal ampulla of vater as the controls. Results The positive rate of Survivin in ampullary carcinomas was significantly higher than that of the controls（82.5% vs 0, P 〈 0. 01 ）. The expression of Survivin in ampullary carcinoma was correlated with duodenal invasion,pancreatic invasion and lymph node metastasis （ P 〈 0.05 ）. Significant difference was also observed in the expression rate of COX-2 between the patients with ampullary carcinoma and the normal controls （67.5% vs 0, P 〈 0. 01 ）. The expression of COX-2 in ampullary carcinoma was correlated with duodenal invasion, pancreatic invasion and lymph node metastasis （ P 〈 0.05 ）. Significantly positive correlation was found between the expression of Survivin and COX-2 by using spearman correlation analysis （ r = 0. 383, P = 0. 015 ）. Conclusion The specific up-regulation of COX-2 gene and Survivin gene may play an important role in the genesis and development of ampullary carcinoma. COX-2 and Survivin may be used as early diagnosis markers and potential therapeutic targets in ampullary carcinoma.

关 键 词：肝胰管壶腹 肿瘤 SURVIVIN 环氧化酶-2 免疫组织化学 

分 类 号：R735.0[医药卫生—肿瘤]