P53、MDM2在鼻咽癌组织中的表达及其与鼻咽癌生物学行为、预后关系的分析研究  

Expression of p53 and MDM2 in Nasopharyngeal Carcinoma and their Correlation with the Biological Behavior and Prognosis of Nasopharyngeal Carcinoma

作　　者：张鹏[1] 吴松珂[2] 徐鹏[1] 冯梅[1] 范子煊[1] 李厨荣[1] 付彬玉[1] 王卫东[1] 郎锦义[1] 

机构地区：[1]四川省肿瘤医院放疗中心,成都610041 [2]什邡市人民医院肿瘤科,四川德阳618400

出　　处：《肿瘤预防与治疗》2012年第5期284-287,共4页Journal of Cancer Control And Treatment

基　　金：四川省科技厅应用基础计划(项目编号:2011JY0109)资助

摘　　要：目的:探讨P53、MDM2在鼻咽癌组织中的表达及与鼻咽癌生物学行为和生存率之间的关系。方法:回顾分析我院2005年～2009年行根治性放化疗治疗的96例鼻咽癌患者,所有患者治疗前行鼻咽部活检病理证实,均采用6MV-X线调强放疗联合以顺铂为主的方案同步化疗,随访3年以上。通过免疫组化SP法检测96例鼻咽癌标本中P53、MDM2蛋白的表达水平,并分析其与临床参数、预后之间关系。结果:P53、MDM2在96例鼻咽癌中阳性表达率分别为65.6%(63/96)、79.17%(76/96)。P53的阳性表达率与不同T分期有关(P=0.000);MDM2在不同N分期的鼻咽癌组织中的表达的差异性具有统计学意义(P=0.001);P53的阳性表达在不同临床分期中的差异性具有统计学意义(P=0.037)。P53与MDM2的表达呈负相关(r=-3.24,P<0.05)。3年无瘤生存率(DFS)及总生存率(OS)分别为73.9%、84.4%。结论:P53、MDMZ的表达与鼻咽癌的生物学行为有关;P53与MDM2的表达呈负相关;P53表达是影响总生存率的预后因素,可以作为评价预后的指标。Objective： To determine the expression of p53 and MDM2 in nasopharyngeal carcinoma and to investigate the relationship between them and biological behavior , survival rate of nasopharyngeal carcinoma. Methods： Clinical data of 96 nasopharyngeal carcinoma patients accepted radical radiotherapy and chemotherapy in our hospital from 2005 -2009 were retrospective analyzed. All patients were diagnosed by biopsy of the nasopharynx before treatment. Patients accepted the 6-MV X-ray intensity-modulated radiotherapy combined with cisplatin-based chemotherapy regimen and were followed up for more than 3 years. The expression levels of P53, MDM2 protein in 96 cases of nasopharyngeal specimens were detected by immunohistochemical SP method and their relationship with the clinical parameters, prognosis were analyzed. Results： The positive expression rate of P53 and MDM2 was 65.6% （63/96）, 79.17% （76/96） respectively. The positive expression rate of P53 was correlated with different T-stage（ P = 0. 000 ）. Expression of MDM2 in different N stage in nasopharyngeal carcinoma had statistical difference（ P = 0. 001 ）. The positive expression of 1＇53 in different clinical stages was statistically different （P = 0. 037）. The expression of 1：＇53 and MDM2 was negatively correlated （r = -3.24,P 〈 0. 05 ）. 3-year Disease-free survival（DFS） and overall survival（OS） of on the patients were 73.9%, 84. 4% respectively. Conclusion： The expression of P53 and MDM2 are related with the biological behavior of nasopharyngeal cancer. The expression of P53 and MDM2 are negatively correlated. P53 can be used as a prognostic factor for overall survival.

关 键 词：P53 MDM2 免疫组织化学法 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤] R730.21[医药卫生—临床医学]