缺氧条件下前列腺上皮细胞体外培养生长类激素受体的表达  

Expression of somatomedin-receptor in anoxic prostate epithelial cells

作　　者：沈文[1] 赵永斌[1] 李萍[1] 黄成[1] 郭飞[1] 吕军[1] 胡卫列[1] 

机构地区：[1]广州军区广州总医院泌尿外科,510010

出　　处：《中国医师进修杂志》2012年第26期1-4,共4页Chinese Journal of Postgraduates of Medicine

基　　金：广东省自然科学基金重点项目（8251001002000001）

摘　　要：目的观察在缺氧条件下前列腺上皮细胞体外培养生长类激素受体表达的变化。方法人前列腺上皮细胞株RWPE-1体外培养。在缺氧或有氧培养环境中分别于接种后4、8、12、24、48h检测前列腺上皮细胞膜表面生长类激素受体：表皮生长因子受体（EGFR）、成纤维细胞生长因子受体（FGFR）、转化生长因子β1受体（TGF-β1R）、胰岛素样生长因子．1受体（IGF-1R）、血管内皮生长因子受体（VEGFR）基因和蛋白表达。基因表达采用RT-PCR法检测，蛋白表达采用免疫组织化学法检测。结果缺氧和有氧培养时EGFR、FGFR、IGF-1R、TGF-B。R、VEGFRmRNA和蛋白的表达均呈上升趋势，差异有统计学意义（P〈0．01）。从各时间点看，两种培养条件除4hEGFRmRNA和12hEGFR蛋白表达，4hIGF-1RmRNA和4、8hIGF．IR蛋白表达，4、8hTGF-13。RmRNA和TGF-β1R蛋白表达，4hVEGFRmRNA表达比较差异无统计学意义（P〉0．05）外，其余各时间点均以缺氧培养时EGFR、FGFR、IGF-1R、TGF-β1R、VEGFRmRNA和蛋白表达高于有氧培养时（P〈0．01）。结论缺氧可刺激前列腺上皮细胞膜表面生长类激素受体表达的上调。Objective To observe the different expression of somatomedin-receptor in cell membrane of prostate epithelial cells at anoxic or normoxic condition. Methods Human prostate epithelial cells line RWPE-1 were cultured in vitro. At 4,8,12,24,48 h after ceils had been seeded, the gene and protein expression of epidermal growth factor receptor （EGFR）, fibroblast growth factor receptor （FGFR）, transforming growth factor 131 receptor （TGF- 13 ,R）, insulin-like growth factor-1 receptor （IGF-1R） and vascular endothelial growth factor receptor （VEGFR） in prostate epithelial cells were tested by RT-PCR and immunohistochem-istry methods, respectively. Results The expression of mRNA and protein of EGFR, FGFR, IGF-1R, TGF- β1R, VEGFR were significantly increased in anoxic and normoxic prostate epithelial cells （P 〈 0.01 ）. At different time point, the expression of mRNA and protein of EGFR, FGFR, IGF-1 R, TGF- β1R, VEGFR significantly higher in anoxic than those in normoxic prostate epithelial cells （P 〈 0.01 ） besides 4 h EGFR mRNA, 12 h EGFR pretein,4 h IGF-1R mRNA, 4 and 8 h IGF-1R protein,4 and 8 h TGF-β1R mRNA, 4 and 8 h TGF-β1R protein,4 h VEGFR mRNA （P〉0.05）. Conclusion Anoxic prostate epithelial cell can up-regulate the expression of somatomedin-receptor.

关 键 词：缺氧 生长调节素类 细胞培养技术 前列腺上皮细胞 

分 类 号：R363[医药卫生—病理学]