有关p16基因在胃癌和结肠癌中缺失状态的研究  

The Vacant Status of p16 in Primary Tumors of Digestive Tract

作　　者：杜爱民[1] 吴国祥[2] 傅伟军 

机构地区：[1]中国人民解放军第85医院,上海200052 [2]中科院上海细胞研究所 [3]第二军医大学附属长征医院

出　　处：《临床消化病杂志》2000年第2期59-61,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：为了研究在多种原发性消化道肿瘤中有无存在ｐ１６基因缺失的状态，采用ＰＣＲ、ＳＳＣＰ分析及ＤＮＡ序列测定技术专门研究了１２例胃癌、结肠癌患者的病理标本。结果表明：①ｐ１６基因缺失的检出率随不同的肿瘤组织类型而异；②分化程度低的消化道肿瘤ｐ１６基因缺失的检出率较高。提示ｐ１６基因的缺失可能与某些原发性恶性消化道肿瘤的发生或发展有关。This study investigated the vacant status of the gene p16 in a variety of primary tumors of digestive tract was carried out in our experiment. In our study of 12 primary tumors of digestive tract (gastric carcinoma, large intestinal cancer), the PCR amplification of DNA and analysis of PCR products were used for determining of gross deletions; the SSCP analysis and DNA sequencing were used for defining of subtle mutations. The results indicated: (1) The incidence of gene p16 deletions varied significantly among different tumor types; (2) The deletions of those lower differentiation tumors of digestive tract were comparatively common and much higher than others. Our study suggested that there are some relationships between the deletions of gene p16 and the emergence or the development of some tumors of digestive tract.

关 键 词：P16基因 胃癌 结肠癌 缺失状态 

分 类 号：R735.2[医药卫生—肿瘤]