γ-干扰素在宫颈上皮内瘤变及宫颈鳞癌中的表达及意义  

The expression and significance of interferon-γ in cervical intraepithelial neoplasia and cervical squamous cell carcinoma

作　　者：郭威[1] 陈瑞萍[1] 黎清[1] 徐建平[1] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510630

出　　处：《暨南大学学报（自然科学与医学版）》2012年第4期387-390,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省医学科研项目(A2006350)

摘　　要：目的:探讨γ-干扰素(γ-Interferon,γ-IFN)在宫颈上皮内瘤变及宫颈鳞癌中的表达及意义。方法:应用免疫组化S-P法检测20例宫颈鳞癌,48例宫颈CIN及10例正常宫颈组织中γ-IFN的表达并应用图像分析技术及阳性单位(positive unit,PU)值表达γ-IFN免疫组化阳性反应的结果。结果:(1)γ-IFN在正常宫颈上皮、宫颈CIN及宫颈鳞癌中表达的PU值分别为(30.80±2.62)、(16.47±1.25)及(9.80±1.62),3组比较P<0.05;(2)在宫颈CINⅠ、Ⅱ、Ⅲ中γ-IFN表达的PU值为(18.17±2.35)、(13.48±1.75)、(10.20±3.54),3组比较P<0.01;(3)在高分化、中分化及低分化宫颈鳞癌中γ-IFN表达的PU值分别为(12.75±1.06)、(9.48±1.28)及(6.06±1.13),3组比较P<0.05;而在Ⅰ、Ⅱ、Ⅲ期宫颈鳞癌中γ-IFN表达的PU值分别为(8.91±0.72)、(8.05±0.77)及(7.79±0.45),3组比较P>0.05。结论:宫颈CIN及宫颈鳞癌的发生与γ-IFN的表达减弱有关;而且宫颈鳞癌细胞的分化程度也与γ-IFN表达下调有关。[ Abstract] Aim：To investigate the expression and significance of ±/-IFN in cervical intraepithelial neo- plasia and cervical squamous cell carcinoma. Methods： S-P immunohistochemistry（IHC）was used to in- vestigate the expression of ±/-IFN in 20 cases of Cervical squamous cell carcinoma,48 cases of Cervical Intraepithelial Neoplasia（CIN）, and 10 cases of normal subjects served as controls. Leica Q550IW soft- ware and PU was used to measure the ±/-IFN expression in different groups. Results： 1. The PU of ±/-IFN positive expression in the controls, CIN and cervical squamous cell carcinoma were （ 30. 80 ± 2. 62 ）, （ 16. 47 ± 1.25 ）, （9.80 ± 1.62） （ P 〈 O. 05 ） ; 2. The PU of y-IFN positive expression in CIN （ CIN I, Ⅱ, Ⅲ ） were （ 18. 17 ± 2. 35 ）, （ 13.48 ± 1.75 ）, （ 10. 20 ± 3.5 ）, such differences among CIN was statis- tically remarkable （P 〈 0. 01 ） ; 3. The expression of y-IFN in various degrees of differentiation of cervi- cal squamous cell carcinoma （ well, moderately and poorly） were （ 12.75 ± 1.06 ）, （ 9. 48 ± 1.28 ）, （6. 06 ± 1.13） （P 〈0. 05） ,but in the FIGO stages of cervical squamous cell carcinoma （ I, Ⅱ, Ⅲsta-ges） were （8.91 ~ 0. 72）, （8.05 ~ 0. 77 ）, （7.79 ~ 0. 45 ）, and these expressions between different sta- ges has no statistically significant （ P 〉 0. 05 ）. Conclusion： The decreased expression of y-IFN in the cervical lesions was associated with occurrence of cervical intraepithelial neoplasia and cervical squamous cell carcinoma, and simuhaneously associated with degrees of differentiation of cervical squamous cell carcinoma.

关 键 词：子宫颈鳞癌 宫颈上皮内瘤变 Γ-干扰素 免疫组织化学 

分 类 号：R737.3[医药卫生—肿瘤]