朗格汉斯细胞与宫颈癌及HPV16E6的关系  

Relationship Among Langerhans' Cells,Cervical Cancer and HPV16 E6

作　　者：江大琼[1] 沈芳[1] 程欣[1] 蔡红兵[1] 

机构地区：[1]武汉大学中南医院妇瘤科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2012年第5期746-748,764,共4页Medical Journal of Wuhan University

摘　　要：目的:研究宫颈组织中朗格汉斯细胞(LC)的数量、形态及分布特点与宫颈癌及人乳头瘤病毒(HPV)16E6的关系。方法:用免疫组织化学技术(SP染色法)对标本的朗格汉斯细胞进行检测,以直接PCR法检测HPV16E6并对E6基因测序。结果:40例宫颈鳞癌,16例宫颈上皮内瘤变(CIN)Ⅱ/Ⅲ级与14例正常宫颈组织中,S-100蛋白阳性LC数量在正常组、CINⅡ/Ⅲ组、宫颈癌组中依次增加(P=0.000),与宫颈癌的临床分期呈负相关(R=-0.552,P=0.000),随宫颈癌组织分化程度降低而减少(P=0.039),与淋巴结转移无关(P=0.245)。HPV16E6在正常对照组、CINⅡ/Ⅲ组和浸润癌组中的阳性率依次上调(P=0.000)。HPV16E6(+)组中LC数量比HPV16E6(-)组少(P=0.011)。LC的表达在HPV16E6原型组和在HPV16E6D25E突变株组中无统计学差异(P=0.813)。结论:LC功能缺陷与宫颈癌的发生有关。Objective： To examine the relationship between Langerhans＇ cells（LCs） and cervical squamous-cell carcinoma（SCC）,and to study the effect of HPV16 E6 on the changes of LCs in normal cervix epithelium,cervical intraepithelia neoplasia（CIN）,and SCC.Methods： The identification of LC was done by immunohistochemical staining on S-100 protein.HPV16 E6 was determined by polymerase chain reaction（PCR） and PCR-directed sequencing.Results： The number of LCs（S-100 positive） was higher in cervical cancer than in normal cervix epithelium（P=0.000）.S-100 protein expression was negatively correlated to the clinical staging of SCC（r=-0.552,P=0.000）,and SCC differentiation（P=0.039）.There was no statistically significant difference,however,between lymphatic metastasis group and lymphatic without metastasis（P=0.245）.The positive rate of HPV16 E6 was 14.3%,56.25%,and 82.5% in normal group,CINⅡ/Ⅲ group and SCC group respectively（P=0.000）.S-100（＋） LCs in HPV16 E6（＋） cases were significant less than in HPV16 E6（-） cases（P=0.011）.No significance difference was found between the number of S-100 LCs in D25E variation group and prototype sequences group（P=0.813）.Conclusion： The defective function of LC was closely correlated to cervical cancer.

关 键 词：宫颈癌 宫颈上皮内瘤变 朗格汉斯细胞 HPV16E6 

分 类 号：R737.33[医药卫生—肿瘤]