二维凝胶电泳结合质谱方法分析日粮组成对奶牛瘤胃乳头蛋白表达的影响  

Effects of Dietary Composition on the Rumen Papillae Tissue Proteome of Cows by the Combination of Two-dimensional Gel Electrophoresis and Mass Spectrometry

作　　者：杨永新[1,2] 王加启[1] 袁廷杰[1] 卜登攀[1] 杨晋辉[1] 张娟霞[1] 周凌云[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室,北京100193 [2]安徽农业科学院畜牧兽医研究所,合肥230031

出　　处：《农业生物技术学报》2012年第9期1042-1047,共6页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展计划(973)项目(No.2011CB100805)

摘　　要：瘤胃是反刍动物营养物质消化吸收的特有器官。为了探索日粮变化对奶牛瘤胃乳头组织蛋白表达的影响,本实验选用24头泌乳日龄和体重一致的初产荷斯坦奶牛(Bos taurus)(其中12头奶牛安装有永久性瘤胃瘘管),随机分为两组,分别饲喂以玉米(Zea mays)秸秆为唯一粗饲料来源的高精料日粮和以羊草(Elymusc hinensis)、苜蓿(Medicago sativa)干草和全株玉米青贮为粗饲料来源的低精料日粮,4周试验期后采集瘤胃乳头,用二维凝胶电泳的蛋白质组学方法分析了奶牛瘤胃乳头蛋白表达的变化。结果发现高精料日粮可造成瘤胃乳头组织表达的乙酰辅酶A合成酶2、羟甲基戊二酰辅酶A合成酶、过氧化物还原酶2和电压依赖性阴离子选择性通道蛋白1等表达量增加。而低精料组奶牛瘤胃乳头组织角蛋白6A和幼体特异性角蛋白(RLK)等蛋白表达量增加。这些鉴定的蛋白涉及代谢、应激及信号转导等功能。结果表明,日粮营养物质的变化可造成奶牛瘤胃乳头蛋白表达量发生改变,组织蛋白的这种变化是适应相应日粮而做出的应答反应。The rumen serves as the primary site for digestion feed and absorption of nutrition, which is the characteristic unique organ presented in ruminant animals. To investigate the changes of rumen papillae proteome affected by dietary change, in the current study, twenty-four primiparous Holstein cows （Bos taurus） with similar day in milk and body weight were randomly assigned to 2 treatments （12 cows fitted with rumenal cannulas）. 1） cows were fed high concentrate diet consisted of corn （Zea mays） stalk and concentrate; 2） cows were fed low concentrate diet consisted of Chinese wildrye （Elymus chinensis）, corn silage, alfalfa（Medicago sativa） hay and concentrate in a 4-wk trial. Rumen papillae was collected t^om ventral rumen wall of cows after a 4-wk trail. Alteration of protein were detected and identified using two-dimensional gel electrophoresis in combination with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results showed that acyl-CoA synthetase family member 2, hydroxymethylglutaryl-CoA synthase, peroxiredoxin-2 and voltage-dependent anion-selective channel protein 1 were upregulated in response to high concentrate diet, while keratin 6A and larva-specific keratin （RLK） were upregulated in response to low concentrate diet. The identified proteins were mainly associated with functions related to stress, metabolism, and signal trasduction. Based on these findings, it was concluded that the changes of rumen papillae proteins affected by dietary composition that mediate rumen epithelial adaptation to dietary change.

关 键 词：二维凝胶电泳 瘤胃 日粮 

分 类 号：S823.91[农业科学—畜牧学]