MDM2与p16在喉癌中的表达及其临床病理意义  

Expression and clinical significance of MDM2 and P16 in laryngeal squamous cell carcinoma

作　　者：李大伟[1] 张庆丰[1] 张欣然[1] 

机构地区：[1]大连市中心医院耳鼻咽喉头颈外科,辽宁大连116033

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第18期802-805,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨喉癌中MDM2和p16基因的表达与喉病变组织的关系,并对MDM2和p16基因在喉癌中表达的关系进行初步研究。方法:应用鼠抗人MDM2单克隆抗体和鼠抗人p16单抗,采用SP免疫组织化学对35例喉鳞状细胞癌(LSCC)、21例喉不典型增生、20例正常喉组织中MDM2蛋白和p16蛋白进行检测。结果:癌周正常喉组织、不典型增生组织和LSCC组织中MDM2蛋白的表达率分别为10.0%、38.1%和54.3%(P<0.05),MDM2蛋白阳性表达率在病理Ⅰ～Ⅲ级肿瘤之间分别为38.1%、66.7%、100.0%,呈明显升高趋势(P<0.05);p16在喉正常组织、不典型增生组织和LSCC组织中的阳性表达率分别为85.1%、52.4%、34.3%(P<0.05),在Ⅰ～Ⅲ级LSCC中的表达分别为52.4%、11.1%和0(P<0.05);MDM2蛋白表达与p16蛋白表达呈负相关(P<0.05)。结论:MDM2蛋白的表达阳性率伴随组织分化程度降低而明显提高,对LSCC诊断有重要辅助意义;p16蛋白的表达伴随组织分化程度的降低而明显降低,在低分化LSCC中不表达,这对于判断LSCC预后有重要意义;MDM2和p16呈相反的表达趋势,两者呈现一定程度的负相关。Abstract Objective：To investigate the relationship between the proteinum expression of MDM2 and P16 in laryngeal squamous cell carcinoma（LSCC）, and their relationship with clinic pathological feature. Method .. The expression of MDM2 and P16 were detected by S-P immunohistochemical method with mouse monoclonal anti MDM2 antibody and anti P16 antibody in 35 LSCC specimens, 21 specimens of precancercerpous larynx, 20 normal laryngeal specimens. Result.. Expression of MDM2 positive in normal laryngeal specimens, precancercerpous larynx and LSCC specimens were 10. 0%, 38. 1%, 54. 3%respectively（P〈0. 05）, and in I-Ⅲ pathological grade laryngeal carcinoma were 38.1% ,66.7%, 100.0% respectively （P〈0.05） Expression of P16 positive in normal laryngeal specimens, precancercerpous larynx and LSCC specimens were 85.1 %, 52.4 %, 34.3 % respectively（P〈0.05）, and in I -Ⅲ pathological grade laryngeal carcinoma were 52.4%, 11.1%, 0% respectively （P〈0.05）. Conclusion.. Expression of MDM2 positive were higher with lower differentiation, so it＇s maybe helpful to diagnose LSCC. Expression of P16 positive were lower with lower differentiation, and therers no expression in HI pathological grade LSCC, so it has positive effect on malignancy determination. Abnormal expression of MDM2 and P16 have different effects on LSCC development. Expression of MDM2 and P16 have negative correlation in Lscc.

关 键 词：喉鳞状细胞癌 MDM2 P16 

分 类 号：R739.65[医药卫生—肿瘤]