利用核糖体工程选育丙酮丁醇菌提高丁醇产量  

Screening of Clostridium strains through ribosome engineering for improved butanol production

作　　者：陈丽杰[1] 商光来[1] 袁文杰[1] 吴又多[1] 白凤武[1] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《生物工程学报》2012年第9期1048-1058,共11页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863计划)(No.2011AA02A208)资助~~

摘　　要：利用核糖体工程技术对丙酮丁醇梭菌Clostridium acetobutylicum L7进行诱变筛选,以获得丁醇高产菌株。使用链霉素诱变C.acetobutylicum L7并结合设计的平板转接逐次提高链霉素浓度的筛选路线,获得丁醇产量较高的菌株S3。结果表明,S3丁醇产量为(12.48±0.03)g/L,乙醇产量为(1.70±0.07)g/L,相对于原始菌分别提高了11.2%及50%;丁醇/葡萄糖转化率由原始菌的0.19提高到0.22,丁醇生产率达到0.24 g/(L.h),相比提高30.5%;耐受丁醇浓度由原始菌的12 g/L提高到14 g/L;发酵液粘度下降到4 mPa/s,同比降低了60%,利于后续分离工作的进行,降低发酵成本。进一步研究工作表明,S3菌株遗传稳定性良好。因此,核糖体工程技术是一种选育丁醇高产菌株的有效方法。We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain $3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in $3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/（L＇h）. The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant ribosome engineering technology may be a promising process strain S3 was also favorable. These results demonstrate that for developing high butanol-producing strains.

关 键 词：核糖体工程 丙酮丁醇梭菌 丁醇发酵 丁醇耐性 链霉素 

分 类 号：TQ923[轻工技术与工程—发酵工程]