改进制作的高光谱免散瞳眼底照相机在眼底病患者临床初步应用验证研究  

Primary study of hyperspectrai non-mydriatic fundus camera prototype on ocular fundus diseases

作　　者：李谐 邹海东[1] 汪枫桦[1] 宫媛媛[1] 曾攀[2] 肖功海[2] 

机构地区：[1]上海交通大学附属第一人民医院眼科,200080 [2]中国科学院上海技术物理研究所

出　　处：《中华眼底病杂志》2012年第5期485-488,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：上海市自然科学基金（10ZR1434300）;上海市人才发展基金（2009006）;上海市眼底病重点实验室开放课题基金（07222911）

摘　　要：目的观察验证改进制作的高光谱免散瞳眼底照相机的性能及眼底病临床检查中的应用价值。方法在Canon免散瞳眼底照相机入射受检眼光路中手动置入窄带滤光片，利用照相机中的面阵电荷耦合器件传感器将所获得图像转换为数字数据，采用高光谱数据分析软件进行图像数据处理。通过12位正常志愿者进行特征波段和仪器测量重复性验证；通过59例接受眼底血管造影的眼底病患者进行特征性高光谱波段下眼底图像检查结果与眼底造影图像的对比分析。结果改进制作的高光谱免散瞳眼底照相机入射受检眼光照度与光功率的最高值均在安全范围内。选取536、547、570、608nm为眼底成像特征光谱，测量仪器重复性测量指标相关性均≥O．85。2例脉络膜新生血管患者608nm图像分析比对结果与眼底造影图像相关系数分别为0．782、0．833。结论改进制作的高光谱免散瞳眼底照相机安全性高，测量结果具有可靠性，特征光谱波段下的眼底照片上可见特征性视网膜和脉络膜解剖结构及病变，能够协助诊断临床疾病，尤其在黄斑病变的诊断中具有良好的应用前景。Objective To observe the performance of hyperspectral non-mydriatic iundus camera prototype and its application on ocular fundus diseases. Methods The narrow hand filters was inserted into the optical path of the Canon non-mydriatic retinal camera （CR-DGi）. The image was converted to digital data by charge-coupled device （CCD）, and then analyzed by hyperspectral data software. Twelve volunteers were examined by hyperspectral non-mydriatic fundus camera prototype to confirm the characteristic wavelength spectrums of ocular fundus diseases and the repeatability of prototype. Fifty-nine patients with ocular fundus diseases who underwent fluoreseein angiography were also examined by hyperspectral non- mydriatic fundus camera prototype, tO compared the images of prototype and fluoreseein angiography. Results Each of the highest power of the light at the focus point and the power per unit were safe. 536, 547, 579 nm were selected as the specific retinal imaging spectrums and 608 nm as the specific choroidal imaging spectrum. The intra-ohserver and inter-observer reproducibility was equal or greater than 0.85. The correlation between hyperspectral non-mydriatic fundus camera prototype and fluorescein angiography in choroidal neovaseularization patients were 0. 782 and 0. 833. Conclusions The hyperspectral non-mydriatic fundus camera prototype is safe and reliable. It shows pathological retinal and choroidal structures with specific spectrums. There are good prospects for the application in clinical diagnosis, especially for macular diseases.

关 键 词：脉络膜新生血管化/诊断 摄影术 诊断显像 

分 类 号：R774[医药卫生—眼科]