荔枝皮原花青素低聚体的定性分析  

Qualitative Analysis of Oligomeric Procyanidins from Litchi chinensis Pericarp

作　　者：周玮婧[1,2] 隋勇[2] 孙智达[2] 谢笔钧[2] 

机构地区：[1]武汉市食品药品检验所,湖北武汉430012 [2]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2012年第17期40-43,共4页Food Science

基　　金：国家自然科学基金-广东省联合基金项目(U0731005);"十一五"国家科技支撑计划项目(2006BAD27B08)

摘　　要：制备并定性分析荔枝皮原花青素低聚体(LPOPC)。荔枝皮原花青素(LPPC)通过乙醇浸提,AB-8大孔树脂纯化,Toyopearl HW-40S树脂层析分级处理得到LPOPC,并按聚合度分成了3个组分。通过红外光谱、高效液相色谱(HPLC)和液相色谱-质谱联用(LC-MS)等分析表明,LPOPC中主要成分为(-)-表儿茶素,并且含有A型原花青素二聚体和A型原花青素三聚体。通过选择性的级分收集,可以得到纯度较高的原花青素单体、二聚体和三聚体。Litchi （Litchi chinensis Sonn.） is a subtropical fruit of high commercial value; however, its pericarp results in a reduced market value. In the present study, oligomeric procyanidins from Litchi chinensis pericarp （LPOPC） were prepared and qualitatively analyzed. LPOPC were extracted with ethanol aqueous solution, purified by AB-8 macroporous resin chromatography, and fractionated by Toyopearl HW-40S resin chromatography to obtain LPOPC. LPOPC was found to comprise three fractions with different degrees of polymerization （DP）. The results of FT-IR, HPLC and LC-MS analyses revealed that the major components of LPOPC were （-）-epicatechin, procyanidin A2, procyanidin A3. Highly pure monomer, dimmers and trimers were obtained through selective collection of fractions.

关 键 词：荔枝皮 原花青素 低聚体 LC-MS 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]