重组腺病毒p53对口腔黏膜异常增生细胞生长的影响  

Effect of recombinant adenovirus-p53 on growth of oral dysplastic keratinocyte

作　　者：张松涛[1] 张媛媛[2] 张壮[3] 李小玉[3] 李龙江[3] 秦建武[1] 

机构地区：[1]河南省肿瘤医院头颈肿瘤外科,郑州450008 [2]郑州大学口腔医院口腔黏膜科 [3]四川大学华西口腔医学院

出　　处：《中国实用医刊》2012年第19期7-10,共4页Chinese Journal of Practical Medicine

摘　　要：目的重组腺病毒p53（rAd—p53）对口腔黏膜异常增生细胞（POE-9n，p53-）生长的影响。方法通过倒置显微镜观察rAd—p53转染前后POE-9n细胞的生长状况及形态变化，并通过流式细胞术及TUNEL荧光染色检测rAd—p53转染后POE-9n细胞凋亡率及其细胞周期分布的改变。结果经rAd—p53转染72h后，POE-9n细胞形态和生长方式发生明显变化并发生凋亡，且具有明显的时间依赖性。转染72h后，POE-9n细胞凋亡最明显，出现明显的细胞周期分布变化，G1期细胞明显增多，S期细胞明显减少，G2期则无明显变化。结论rAd—p53可以明显抑制POE-9n细胞的增殖，调节细胞周期，使细胞停滞在G1期，并诱导细胞产生凋亡。Objective To investigate the effect of recombinant adenovirus-p53 （rAd-p53） on growth of oral dysplastic keratinocyte（POE-9n, p53 - ）. Methods Inverted microscope was used to detect the growth status and morphologic changes of POE-9n cell before and after transfeetion. The cell cycle change and apoptotic rates of POE-9n cell line were analyzed by flow cytometry （FCM） and TUNEL assay. Results After rAd-p53 infection for 72 h, typical morphologic changes were observed in POE-9n cells. By FCM and TUNEL, the time-dependent induced apoptosis was examined. After rAd-p53 infection for 72 h, it was most obvious and induced the arrest cell cycle in G1 stage accompanied with the decrease of S stage cells and no change in G2 stage cells. Conclusions tAd-p53 can strongly inhibit cell proliferation and induce apoptosis and arrest cell cycle in G1 of POE-9n cells.

关 键 词：腺病毒 癌前病变 基因治疗 凋亡 细胞周期 

分 类 号：R78[医药卫生—口腔医学]