镉金属硫蛋白对小鼠脾淋巴细胞DNA链损伤和修复的体外实验研究  

IN VITRO STUDY ON THE DNA INJURIES AND REPAIR OF SPLENIC LYMPHOCYTE INDUCED BY CADMIUM-METALLOTHIONEIN

作　　者：林忠宁[1] 董胜璋[1] 蔡颖[1] 董书芸[1] 余贵英[1] 任铁玲[1] 

机构地区：[1]中山医科大学公共卫生学院环境卫生学教研室,广东广州510089

出　　处：《癌变．畸变．突变》2000年第2期72-75,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金!(39770 6 36 )

摘　　要：目的 :为研究外源性镉金属硫蛋白 (CdMT)对淋巴细胞DNA的损伤和修复作用。方法 :采用单细胞电泳法 (SCGE)检测DNA单链断裂 (SSBs) ,观察在脱金属硫蛋白 (Apo -MT)和不同终浓度CdMT(0 .1μmolCd2 +/L、1.0 μmolCd2 +/L、10 .0 μmolCd2 +/L、2 0 .0 μmolCd2 +/L)染毒作用 30分钟和 6 0分钟时DNA链损伤 ,和去除受试物后 30分钟、6 0分钟、12 0分钟时DNA链损伤的修复。结果 :DNA -SSBs尾长在Apo -MT作用未见增加 ;在中、高剂量CdMT染毒 30和 6 0分钟时都明显增长。损伤后链修复DNA迁移尾长 ,在中、高剂量CdMT组都显著低于细胞染毒时 ,但明显高于对照和Apo -MT组。 结论 :提示CdMT直接作用可致脾淋巴细胞DNA链的损伤 。Purpose: To study and compare DNA injuries and repair in BALB/C mice splenic lymphocyte induced by exogenous cadmium metallothionein(CdMT). Methods:Single cell gel electrophoresis (SCGE) assay was used to detect DNA single strand breaks (DNA SSBs) when lymphocyte were induced with different concentration of CdMT for 30 or 60 minutes, and the ability of DNA repair after CdMT was removed for different times from 30 to 120 minutes respectively. Results: It was showed that the comet length of DNA SSBs did not found change in Apo MT group, while there were significant increasement of the comet length in mid or high dose CdMT group at both exposure times. And the comet length of each DNA repair times(30 to 120 min) in mid or high dose CdMT group was significantly shorter than that of DNA injuries, but longer than that of control and Apo MT group. Conclusion: It is indicated that lymphocyte DNA SSBs damage would be induced by CdMT exposed directly, and that there were repair activity for DNA SSBs in spleen lymphocyte in vitro.

关 键 词：金属硫蛋白 镉 DNA损伤 修复 单细胞电泳 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤] R995[医药卫生—临床医学]