慢性HBV感染时血清可溶性Fas浓度的变化及临床意义  

Level of serum sFas and its clinical significance in chronic HBV infection patients

作　　者：魏红山[1] 陆伟君[2] 李定国[1] 陆汉明[1] 王延军[2] 劳平[2] 

机构地区：[1]上海第二医科大学附属新华医院,上海200092 [2]山东省立医院,山东250000

出　　处：《胃肠病学和肝病学杂志》2000年第1期11-13,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的　观察HBV复制状态对肝细胞Fas分子表达的影响 ,探讨慢性HBV感染时血清可溶性Fas分子 (sFas)浓度的变化及可能的临床意义。方法　 81例组织标本为慢性肝炎患者肝穿刺标本 (慢性活动性肝炎 19例 ,慢性迁延性肝炎 62例 ) ,患者血清标本于 -70℃冷藏。肝组织标本Fas分子用免疫组化LSAB法检测 ,肝细胞凋亡的检测用DNA末端标记法 (TUNEL法 )。血清HBVDNA用PCR法检测 ,血清sFas浓度用ELISA法检测。结果　肝组织Fas分子表达强度与HBV复制有关 ,血清HBVDNA阳性组明显高于阴性组 (P <0 0 1) ,血清sFas浓度 (ng/mL)与肝组织炎症程度有关 ,慢性活动性肝炎明显高于慢性迁延性肝炎 ( 4 72± 1 2 4vs 2 5 2± 0 94,P <0 0 1)。结论　肝细胞Fas分子的表达与HBV复制程度有关 。Aim To investigate serum level of soluble Fas (sFas) and its clinical significance in chronic HBV infection patients.Method Fas was detected by immnohistochemistry (LSAB) and apoptosis by TUNEL method in 81 liver specimens of patients acquired by biopsy.Their serum HBV DNA was detected by PCR and level of serum sFas by ELISA.Results Intensity of Fas expression in liver was correlated with replication of HBV and the positive rate of Fas expression was higher in HBV DNA positive patients than that in negative patients (P<001)Level of serum sFas was correlated with degree of liver inflammation and higher in chronic active hepatitis (CAH) than that in chronic persistent hepatitis (CPH) (P<001).Conclusion Fas expression was related to HBV replication and serum sFas was helpful for judging the degree of hepatocyte lesion.

关 键 词：凋亡 慢性 肝炎 诊断 可溶性FAS 

分 类 号：R575.1[医药卫生—消化系统]