采用单引物AP-PCR技术对胃癌相关基因片段的分离分析  

Isolation and analysis of gastric cancer associated DNA fragments by using single primer AP-PCR

作　　者：胡蓉[1] 易粹琼[1] 王家龙[1] 

机构地区：[1]同济医科大学协和医院消化科,武汉430022

出　　处：《胃肠病学和肝病学杂志》2000年第1期45-47,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的　分离与胃癌发生相关的基因片段并予以鉴定。方法　运用单引物AP -PCR技术 ,对 2 0例配对的胃癌组织和非癌胃组织的基因DNA进行扩增 ,分离出差异片段 ,进行克隆 ,测序和杂交鉴定。结果　分离出三条差异片段 ,其中一条片段 ( 8ndf)基因文库内无同源序列存在 ,斑点杂交显示该片段存在于非癌组织中而癌组织中缺如。结论　单引物AP -PCR技术对于分离差异基因行之有效 ;Aim Isolation and identification of differentially expressed DNA fragments from matched human gastric cancer and non-cancerous gastric tissues.Methods Differential DNA fragments were isolated by using single primer AP-PCR from 20 patients with gastric cancer.The DNA fragmentes were cloned,sequenced and hybridized.Results Three DNA fragments were isolated.It was showed there was no homologous sequence of 8ndf in GeneBank.Dot blot hybridization revealed that this gene appeared in non-cancer tissues rather than cancer tissues.Conclusions Single primer AP-PCR is an effective method for isolating differentially expressed genes.The 8ndf might be a new tumor suppressor gene.

关 键 词：胃癌 基因 AP-PCR 斑点杂交 

分 类 号：R735.2[医药卫生—肿瘤]